神经胶质细胞培养物中前列腺素受体信使核糖核酸的表达模式

Expression pattern of messenger RNAs for prostanoid receptors in glial cell cultures.

作者信息

Kitanaka J, Hashimoto H, Gotoh M, Kondo K, Sakata K, Hirasawa Y, Sawada M, Suzumura A, Marunouchi T, Matsuda T, Baba A

机构信息

Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.

出版信息

Brain Res. 1996 Jan 29;707(2):282-7. doi: 10.1016/0006-8993(95)01256-7.

DOI:10.1016/0006-8993(95)01256-7

PMID:8919306

Abstract

Expression level of messenger RNAs (mRNAs) for prostanoid EP3, FP, and TP receptors was investigated in cultured rat astrocytes, oligodendrocytes, and microglia, as well as in meningeal fibroblasts, rat glioma C6 cells, rat pheochromocytoma PC12 cells, whole brain, and several peripheral tissues by reverse transcriptase-polymerase chain reaction. Cultured astrocytes and oligodendrocytes expressed mRNAs for 3 prostanoid receptors examined. In contrast, cultured microglia and pheochromocytoma PC12 cells expressed EP3 and TP receptor mRNAs, but not FP receptor mRNA. Glioma C6 cells expressed only TP receptor mRNA among 3 prostanoid receptors with the same expression level as that in astrocytes. Cultured meningeal fibroblasts expressed 3 receptor transcripts, and their expression levels were lower than those in astrocytes. Expression level of mRNA for each prostanoid receptor in cultured glial cells was higher than that in whole brain. These observations suggest that each prostanoid has its specific roles in each glial cell type of the brain.

摘要

通过逆转录聚合酶链反应，研究了前列腺素EP3、FP和TP受体的信使核糖核酸（mRNA）在培养的大鼠星形胶质细胞、少突胶质细胞和小胶质细胞，以及脑膜成纤维细胞、大鼠胶质瘤C6细胞、大鼠嗜铬细胞瘤PC12细胞、全脑和几种外周组织中的表达水平。培养的星形胶质细胞和少突胶质细胞表达所检测的3种前列腺素受体的mRNA。相比之下，培养的小胶质细胞和嗜铬细胞瘤PC12细胞表达EP3和TP受体mRNA，但不表达FP受体mRNA。胶质瘤C6细胞在3种前列腺素受体中仅表达TP受体mRNA，其表达水平与星形胶质细胞中的相同。培养的脑膜成纤维细胞表达3种受体转录本，其表达水平低于星形胶质细胞中的表达水平。培养的神经胶质细胞中每种前列腺素受体的mRNA表达水平高于全脑中的表达水平。这些观察结果表明，每种前列腺素在大脑的每种神经胶质细胞类型中都有其特定作用。