Beumer R R, te Giffel M C, Spoorenberg E, Rombouts F M
Agricultural University, Laboratory of Food Microbiology, Wageningen, The Netherlands.
Epidemiol Infect. 1996 Dec;117(3):437-42. doi: 10.1017/s0950268800059094.
Using a direct isolation method Listeria spp. were detected in 101 (47.4%) of 213 houses investigated. L. monocytogenes was present in 45 houses (21.1%). Listeria spp. occurred at all sampling sites. Dish-cloths (37%) and surface samples round the drain in the bathroom (27.2%) were most frequently contaminated. Highest numbers (c. 10(4) c.f.u./object) were found in dish-cloths and washing-up brushes. Lower levels (up to 10(3) c.f.u./object) were obtained from kitchen sinks, refrigerator vegetable compartment samples and tooth brushes. In total, 132 isolations of Listeria spp. were made from 871 samples. L. innocua (53%) and L. monocytogenes (41%) were the predominant species in the positive samples. Other Listeria spp. were found in only 6% of the positive samples.
采用直接分离法,在调查的213所房屋中的101所(47.4%)检测到李斯特菌属。45所房屋(21.1%)中存在单核细胞增生李斯特菌。李斯特菌属出现在所有采样点。抹布(37%)和浴室排水口周围的表面样本(27.2%)受污染最为频繁。在抹布和洗碗刷中发现的菌数最高(约10⁴ 菌落形成单位/物品)。从厨房水槽、冰箱蔬菜隔层样本和牙刷中获得的菌数较低(最高10³ 菌落形成单位/物品)。总共从871个样本中分离出132株李斯特菌属。无害李斯特菌(53%)和单核细胞增生李斯特菌(41%)是阳性样本中的主要菌种。仅6%的阳性样本中发现了其他李斯特菌属菌种。