胆红素对肺表面活性物质表面张力特性的影响。

Influence of bilirubin on surface tension properties of lung surfactant.

作者信息

Amato M, Schürch S, Grunder R, Bachofen H, Burri P H

机构信息

Institute of Anatomy, University of Berne, Switzerland.

出版信息

Arch Dis Child Fetal Neonatal Ed. 1996 Nov;75(3):F191-6. doi: 10.1136/fn.75.3.f191.

DOI:10.1136/fn.75.3.f191

PMID:8976686

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1061199/

Abstract

AIM

To investigate the influence of bilirubin on the surface tension activity of a porcine derived (Curosurf) and synthetic (Exosurf) surfactant.

METHODS

The captive bubble surfactometer at phospholipid doses of 0.5 mg/ml (low dose) and 1 mg/ml (high dose) in solutions of increasing bilirubin concentrations (0.25, 0.5, and 1.0 mg/ml) was used.

RESULTS

Curosurf (without bilirubin) showed a higher surface f1p4ion activity than Exosurf, as shown by area compression of 30 (SD 0.6)% compared with 76(1.4)% at low surfactant dose and 25 (0.9)% compared with 85 (0.5)% at high dose (P < 0.01). Bilirubin showed negligible surface activity at the concentrations studied. At low phospholipid dose (0.5 mg/ml Curosurf), bilirubin increased film area compression of lipid extract surfactant from 30 (0.6)% to 55 (1.6)%, 59 (0.1)%, and 68 (0.5)% at the three studied bilirubin concentrations, respectively (P < 0.01). At high phospholipid dose (1 mg/ml Curosurf), bilirubin had the same adverse, although less pronounced, effect on film area compression of porcine lipid extract surfactant (25 (0.9)% vs 26 (0.9)%, 39 (1.3)%, and 44 (1.1)%, respectively) (P < 0.01). Using synthetic surfactant (Exosurf), with a much lower original surface activity, bilirubin did not further inhibit its surface tension properties at any of the phospholipid doses studied.

CONCLUSION

These results indicate that in vitro bilirubin impairs the surface tension activity of porcine lipid extract surfactant, but does not affect synthetic surfactant activity.

摘要

目的

研究胆红素对猪源性（珂立苏）和合成性（固尔苏）表面活性剂表面张力活性的影响。

方法

使用俘获气泡表面张力仪，在胆红素浓度递增（0.25、0.5和1.0mg/ml）的溶液中，磷脂剂量为0.5mg/ml（低剂量）和1mg/ml（高剂量）。

结果

珂立苏（不含胆红素）显示出比固尔苏更高的表面活性，低表面活性剂剂量下面积压缩率为30（标准差0.6）%，而固尔苏为76（1.4）%；高剂量下分别为25（0.9）%和85（0.5）%（P<0.01）。在所研究的浓度下，胆红素的表面活性可忽略不计。在低磷脂剂量（0.5mg/ml珂立苏）时，胆红素使脂质提取物表面活性剂的膜面积压缩率在三个研究的胆红素浓度下分别从30（0.6）%增加到55（1.6）%、59（0.1）%和68（0.5）%（P<0.01）。在高磷脂剂量（1mg/ml珂立苏）时，胆红素对猪脂质提取物表面活性剂的膜面积压缩率有相同的不良影响，尽管不太明显（分别为25（0.9）%对26（0.9）%、39（1.3）%和44（1.1）%）（P<0.01）。使用合成表面活性剂（固尔苏），其原始表面活性低得多，在任何研究的磷脂剂量下，胆红素均未进一步抑制其表面张力特性。