nfi，大肠杆菌K-12中核酸内切酶V的基因。
nfi, the gene for endonuclease V in Escherichia coli K-12.
作者信息
Guo G, Ding Y, Weiss B
机构信息
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0602, USA.
出版信息
J Bacteriol. 1997 Jan;179(2):310-6. doi: 10.1128/jb.179.2.310-316.1997.
Abstract
Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal sequence, its gene, nfi, was identified. nfi is immediately downstream of hemE, at kb 4208 (90.4 min) on the current chromosomal map of Escherichia coli K-12. This region was cloned, and plasmid insertion and deletion mutants were used to study its molecular organization. Although nfi is the third of four closely spaced, codirectional genes, it is expressed independently.
摘要
核酸内切酶V对单链DNA或含有尿嘧啶或被多种因子损伤的双链DNA具有特异性（B. 登普尔和S. 林恩，《生物化学杂志》257:2848 - 2855，1982）。因此，它可能是一种多功能的DNA修复酶。该蛋白质被纯化至表观均一，根据其N端序列鉴定出了它的基因nfi。nfi紧挨着hemE下游，在大肠杆菌K - 12当前染色体图谱上的4208 kb（90.4分钟）处。对该区域进行了克隆，并使用质粒插入和缺失突变体来研究其分子结构。虽然nfi是四个紧密相邻、同向排列的基因中的第三个，但它是独立表达的。
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