从西班牙腹泻山羊羔中分离出的大肠杆菌菌株的血清型、毒素及抗生素耐药性

Serogroups, toxins and antibiotic resistance of Escherichia coli strains isolated form diarrhoeic goat kids in Spain.

作者信息

Cid D, Blanco M, Blanco J E, Ruíz Santa Quiteira J A, de la Fuente R, Blanco J

机构信息

Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.

出版信息

Vet Microbiol. 1996 Dec;53(3-4):349-54. doi: 10.1016/s0378-1135(96)01222-9.

DOI:10.1016/s0378-1135(96)01222-9

PMID:9008345

Abstract

Fifty-five Escherichia coli strains isolated from 55 diarrhoeic goat kids from 13 flocks in Spain were serotyped and investigated for production of enterotoxins (LT and STa), verotoxins (VT1 and VT2), cytotoxic necrotizing factors (CNF1 and CNF2), alpha-hemolysin (Hly) and enterohemolysin (EntHly), and for antibiotic resistance. Only 3 (5%) strains were toxigenic: 1 VT1+EntHly+ (serogroup O8) and 2 CNF2+ (both of serogroup O153). The strains serotyped belonged to 19 serogroups. However, 31 (56%) were of one nine serogroups (O3, O8, O9, O10, O11, O21, O44, O103 and O153) and only three of them (O8, O9 and O11) accounted for 29% of the strains. The highest percentages of antibiotic resistance in order of frequency were: streptomycin (93%), sulfadiazine (89%), tetracyline (84%), kanamycin (82%), neomycin (82%) and ampicillin (69%). We conclude that E. coli strains isolated from diarrhoeic goat kids are usually non-toxigenic and belong to a large number of serogroups.

摘要

对从西班牙13个羊群中55只腹泻山羊羔分离出的55株大肠杆菌进行了血清分型，并检测了其肠毒素（LT和STa）、维罗毒素（VT1和VT2）、细胞毒性坏死因子（CNF1和CNF2）、α-溶血素（Hly）和肠溶血素（EntHly）的产生情况以及抗生素耐药性。只有3株（5%）菌株产毒：1株VT1+EntHly+（血清群O8）和2株CNF2+（均为血清群O153）。所分型的菌株属于19个血清群。然而，31株（56%）属于9个血清群（O3、O8、O9、O10、O11、O21、O44、O103和O153），其中只有3个血清群（O8、O9和O11）占菌株总数的29%。抗生素耐药率从高到低依次为：链霉素（93%）、磺胺嘧啶（89%）、四环素（84%）、卡那霉素（82%）、新霉素（82%）和氨苄西林（69%）。我们得出结论，从腹泻山羊羔分离出的大肠杆菌菌株通常不产毒，且属于大量血清群。