在马白细胞中检测到马疱疹病毒1型的潜伏相关转录本，但在三叉神经节中未检测到。
Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia.
作者信息
Chesters P M, Allsop R, Purewal A, Edington N
机构信息
Department of Pathology and Infectious Diseases, The Royal Veterinary College, London, United Kingdom.
出版信息
J Virol. 1997 May;71(5):3437-43. doi: 10.1128/JVI.71.5.3437-3443.1997.
Abstract
Results from Southern hybridization and PCR amplification experiments using a randomly synthesized reverse transcription-PCR product showed that peripheral blood leukocytes from horses showing no clinical signs of disease expressed a putative latency-associated transcript antisense to and overlapping the 3' end of the equid herpesvirus 1 (EHV-1) immediate-early gene (gene 64). A PCR product derived from this transcript has > or =96% identity with the published EHV-1 sequence. In situ hybridization studies of equine bronchial lymph nodes corroborated these findings and are consistent with reactivation data (D. A. Smith, A. Hamblin, and N. Edington, unpublished data), indicating that EHV-1 latency is established predominantly in CD5+/CD8+ leukocytes.
摘要
使用随机合成的逆转录 - PCR产物进行的Southern杂交和PCR扩增实验结果表明，来自未表现出疾病临床症状的马匹的外周血白细胞表达了一种假定的潜伏相关转录本，该转录本与马疱疹病毒1型（EHV - 1）立即早期基因（基因64）的3'端反义且重叠。源自该转录本的PCR产物与已发表的EHV - 1序列具有≥96%的同一性。马支气管淋巴结的原位杂交研究证实了这些发现，并且与再激活数据一致（D. A. Smith、A. Hamblin和N. Edington，未发表数据），表明EHV - 1潜伏主要在CD5 + / CD8 +白细胞中建立。
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