针对补体调节蛋白的单克隆抗体的流式细胞术和免疫印迹分析。

Flow cytometry and immunoblotting analysis of monoclonal antibodies directed to complement regulatory proteins.

作者信息

Blanchard D, Navenot J M, Petit-Le Roux Y, Willem C, Loirat M J

机构信息

Etablissement de Transfusion Sanguine de Loire Atlantique/Vendée, Site de Nantes, France.

出版信息

Transfus Clin Biol. 1997;4(1):131-4. doi: 10.1016/s1246-7820(97)80028-6.

DOI:10.1016/s1246-7820(97)80028-6

PMID:9095519

Abstract

A series of 18 monoclonal antibodies directed to complement regulatory proteins were investigated by flow cytometry and immunoblotting. Seventeen antibodies are directed against a phosphatidyl inositol-linked glycoprotein since they show a dual population of erythrocytes from a paroxysmal nocturnal haemoglobinuria (PNH) patient. From this group, 6 antibodies revealed a 65-70 kDa band on immunoblots allowing their identification as anti-DAF (Decay Accelerating Factor; CD55 antigen), and 11 bound to a 20 kDa molecule corresponding to MIRL (Membrane Inhibitor of Reactive lysis, CD59 antigen). One antibody revealed an homogeneous population from the PNH patient and bound to a 200 kDa band on immunoblot that might corresponds to the CR1 (Complement Receptor type 1; CD35).

摘要

通过流式细胞术和免疫印迹法对一系列18种针对补体调节蛋白的单克隆抗体进行了研究。17种抗体针对磷脂酰肌醇连接糖蛋白，因为它们在阵发性夜间血红蛋白尿（PNH）患者的红细胞中显示出双群体。在该组中，6种抗体在免疫印迹上显示出65 - 70 kDa条带，使其被鉴定为抗衰变加速因子（DAF；CD55抗原），11种抗体与对应于膜反应性溶解抑制物（MIRL；CD59抗原）的20 kDa分子结合。一种抗体在PNH患者中显示出均匀群体，并在免疫印迹上与可能对应于补体受体1型（CR1；CD35）的200 kDa条带结合。