Bram S, Baudy P, Lepault J, Hermann D
Nucleic Acids Res. 1977 Jul;4(7):2275-82. doi: 10.1093/nar/4.7.2275.
Intact chromatin, chromatin minus histone H1, and nuclease digestion fragments have been studied by very small angle neutron scattering. The results are not consistent with a straight chain of nucleosomes and require the presence of a higher order coiling in monovalent salt solutions. The data are interpretable by a structure having a cross section radius of gryration of 8.5 +/- 1 nm, which suggests an outer diameter for a coil of nucleosomes of 27 +/- 3 nm.
完整染色质、去除组蛋白H1的染色质以及核酸酶消化片段已通过极小孔角中子散射进行了研究。结果与核小体的直链结构不一致,并且需要在单价盐溶液中存在更高阶的盘绕。这些数据可以用回转半径为8.5±1纳米的结构来解释,这表明核小体线圈的外径为27±3纳米。