接受促肾上腺皮质激素治疗的多发性硬化症患者的红细胞超氧化物歧化酶和过氧化氢酶活性

Red blood cell superoxide dismutase and catalase activities in patients suffering from multiple sclerosis treated with adrenocorticotropic hormone.

作者信息

Kopff M, Zakrzewska I, Czernicki J, Klem J, Strzelczyk M, Chmielewski H

机构信息

Department of Biochemistry, Military Medical Academy, Lódź, Poland.

出版信息

Pol J Pharmacol. 1996 Jul-Aug;48(4):441-5.

PMID:9112685

Abstract

The purpose of this study was to determine superoxide dismutase (SOD) and catalase (CAT) activities in erythrocytes of patients with multiple sclerosis treated with ACTH. SOD activity in hemolysates was determined according to the method of Misra and Fridovich and calculated as units per gram of hemoglobin (Hb). CAT activity in hemolysates was determined with Beers and Sizer's method and expressed in IU/g Hb. SOD activity in control group was (1.61 +/- 0.45) x 10(3) U/g Hb whereas, the activity of CAT amounted to (5.88 +/- 1.36) x 10(4) U/g Hb. Before the treatment, SOD activity was decreased by approximately 20% ((1.25 +/- 0.25) x 10(3) U/g Hb) while that of CAT-by about 7.7% ((5.43 +/- 0.68) x 10(4) U/g Hb) in comparison to the normal control. After treatment with ACTH, activity of both enzymes increased: SOD-by about 34.4% to (1.68 +/- 0.38) x 10(3) U/g Hb and CAT-by about 7% to (6.29 +/- 0.55) x 10(4) U/g Hb. Results of investigations showed that ACTH caused an increase in CAT and SOD activities in erythrocytes of patients after three-week treatment.

摘要

本研究的目的是测定接受促肾上腺皮质激素（ACTH）治疗的多发性硬化症患者红细胞中的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。溶血产物中的SOD活性根据米斯拉（Misra）和弗里多维奇（Fridovich）的方法测定，并以每克血红蛋白（Hb）的单位数计算。溶血产物中的CAT活性采用比尔斯（Beers）和西泽（Sizer）的方法测定，并以国际单位/克Hb表示。对照组的SOD活性为（1.61±0.45）×10³U/g Hb，而CAT活性为（5.88±1.36）×10⁴U/g Hb。治疗前，与正常对照组相比，SOD活性降低了约20%（（1.25±0.25）×10³U/g Hb），而CAT活性降低了约7.7%（（5.43±0.68）×10⁴U/g Hb）。用ACTH治疗后，两种酶的活性均升高：SOD升高约34.4%至（1.68±0.38）×10³U/g Hb，CAT升高约7%至（6.29±0.55）×10⁴U/g Hb。研究结果表明，ACTH在为期三周的治疗后使患者红细胞中的CAT和SOD活性增加。