Stiernstedt S H, Wretlind B
Karolinska Institutet, Department of Infectious Diseases, Danderyd, Sweden.
Antimicrob Agents Chemother. 1996 Dec;40(12):2882-3. doi: 10.1128/AAC.40.12.2882.
A constant benzylpenicillin (penicillin G) concentration for determination of the MIC for strains of Borrelia burgdorferi was achieved by dialysis culture. The strains were grown in dialysis membrane bags with daily transfer to tubes with freshly added benzylpenicillin. The MICs decreased by one or several dilution steps compared with the conventional procedure.
通过透析培养获得用于测定伯氏疏螺旋体菌株最低抑菌浓度(MIC)的恒定苄青霉素(青霉素G)浓度。将菌株培养于透析膜袋中,每天转移至添加新鲜苄青霉素的试管中。与传统方法相比,最低抑菌浓度降低了一个或几个稀释步骤。