小鼠平滑肌肌球蛋白重链的分子克隆与表达

Molecular cloning and expression of murine smooth muscle myosin heavy chains.

作者信息

Hasegawa K, Arakawa E, Oda S, Matsuda Y

机构信息

Vessel Research Laboratory, Co., Ltd., Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1997 Mar 17;232(2):313-6. doi: 10.1006/bbrc.1997.6281.

DOI:10.1006/bbrc.1997.6281

PMID:9125171

Abstract

The nucleotide and deduced amino acid sequences of two isoforms of mouse smooth-muscle myosin heavy chain (SM1 and SM2) were determined. SM1 (6175 bp) and SM2 (6214 bp) cDNA contained a single open reading frame that encodes 1972 and 1938 amino acids (227,056 Da and 223,294 Da), respectively. Smooth muscle myosin heavy chain mRNA was expressed highly in smooth muscle tissue (small intestine) and weakly in heart and lung. Each of SM1 and SM2 cDNA was transfected and expressed in CHO cells. The expressed myosin heavy chains were detected with an antibody raised against smooth muscle myosin heavy chains and showed the same mobility as the native smooth muscle myosin heavy chains in SDS-PAGE.

摘要

测定了小鼠平滑肌肌球蛋白重链两种同工型（SM1和SM2）的核苷酸序列和推导的氨基酸序列。SM1（6175bp）和SM2（6214bp）的cDNA包含一个单一的开放阅读框，分别编码1972和1938个氨基酸（227,056Da和223,294Da）。平滑肌肌球蛋白重链mRNA在平滑肌组织（小肠）中高表达，在心脏和肺中低表达。将SM1和SM2的cDNA分别转染并在CHO细胞中表达。用抗平滑肌肌球蛋白重链的抗体检测表达的肌球蛋白重链，其在SDS-PAGE中的迁移率与天然平滑肌肌球蛋白重链相同。