小鼠RT6基因座1和大鼠RT6.2是NAD⁺。具有自动ADP-核糖基化活性的精氨酸ADP-核糖基转移酶。

Mouse RT6 locus 1 and rat RT6.2 are NAD+. Arginine ADP-ribosyltransferases with auto-ADP-ribosylation activity.

作者信息

Bortell R, Rigby M, Stevens L, Moss J, Kanaitsuka T, Mordes J, Greiner D, Rossini A

机构信息

Diabetes Division, University of Massachusetts Medical School, Worcester 01605, USA.

出版信息

Adv Exp Med Biol. 1997;419:169-73.

PMID:9193650

Abstract

We report that rat RT6.2 and recombinant mouse Rt6 locus 1 proteins possess auto-ADP-ribosyltransferase activity and that Rt6, but not RT6, catalyzes the ADP-ribosylation of exogenous histones. Based on NH2OH sensitivity, it appeared that the ADP-ribose was attached to arginine residues on proteins. We also observed that the NAD+ concentration in culture medium correlates inversely with the proliferation of rat RT6+ T cells. The data suggest that lymphocyte surface ADP-ribosyltransferases could be involved in signaling and immunoregulatory processes.

摘要

我们报告称，大鼠RT6.2和重组小鼠Rt6基因座1蛋白具有自动ADP-核糖基转移酶活性，且Rt6（而非RT6）催化外源性组蛋白的ADP-核糖基化。基于对NH2OH的敏感性，似乎ADP-核糖连接到了蛋白质上的精氨酸残基。我们还观察到，培养基中的NAD+浓度与大鼠RT6+ T细胞的增殖呈负相关。这些数据表明，淋巴细胞表面的ADP-核糖基转移酶可能参与信号传导和免疫调节过程。