Davis J F, de la Torre J C, Castro A E, Connolly B M, Doman J T, Dunn P A
Georgia Poultry Laboratory, Oakwood 30566, USA.
Avian Dis. 1997 Apr-Jun;41(2):442-6.
Severe hypoglycemia-spiking mortality syndrome was experimentally reproduced in broiler chicks. Inoculum was homogenized brains from 28-day-old commercial broiler chicks with central nervous system signs (50% [v/v] in phosphate-buffered saline with 2% fetal calf serum). Oral inoculations of 1.2 ml of the homogenate were given at 1 day of age to broiler chicks (n = 15). Fourteen days later, chicks were fasted and stressed with a 2-sec cool water spray. Six chicks (40%) developed clinical signs of spiking mortality syndrome and were severely hypoglycemic. Uninoculated control chicks (n = 15) from the same hatch, also fasted and stressed simultaneously, were unaffected. Examination of a banded fraction produced from the inoculum with the use of transmission electron microscopy with negative staining revealed viruslike particles indistinguishable from arenavirus particles stained and examined simultaneously. Avian encephalomyelitis virus was isolated by one of three laboratories attempting virus isolation with the use of embryonating chicken eggs.
在肉鸡雏鸡中通过实验再现了严重低血糖性猝死综合征。接种物是来自患有中枢神经系统症状的28日龄商业肉鸡雏鸡的匀浆脑（在含2%胎牛血清的磷酸盐缓冲盐水中为50%[v/v]）。在1日龄时给肉鸡雏鸡（n = 15）口服接种1.2 ml匀浆。14天后，雏鸡禁食并用2秒的冷水喷雾使其应激。6只雏鸡（40%）出现了猝死综合征的临床症状且严重低血糖。来自同一孵化批次的未接种对照雏鸡（n = 15），也同时禁食并应激，未受影响。使用负染色的透射电子显微镜检查接种物产生的条带部分，发现了与同时染色和检查的沙粒病毒颗粒难以区分的病毒样颗粒。三个尝试使用鸡胚进行病毒分离的实验室中有一个分离出了禽脑脊髓炎病毒。
