Pybus V, Onderdonk A B
Channing Laboratory, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
J Infect Dis. 1997 Feb;175(2):406-13. doi: 10.1093/infdis/175.2.406.
Six strains of Prevotella bivia and 4 of Gardnerella vaginalis were examined for nutrient substrate utilization as part of ongoing studies on the pathogenesis of bacterial vaginosis. Addition of single amino acids to vaginal defined medium (VDM) was stimulatory to the growth of P. bivia but not to G. vaginalis. However, peptides significantly promoted the growth of both organisms. Growth of P. bivia in VDM and VDM supplemented with either amino acids or peptone was accompanied by net ammonia production, while growth of G. vaginalis under the same conditions resulted in net ammonia utilization. Ammonia-enriched supernatants from the growth of P. bivia in peptone-supplemented VDM were stimulatory to G. vaginalis growth. However, ammonia-reduced supernatants from G. vaginalis growth in peptone-supplemented VDM had a neutral effect on P. bivia growth. A commensal relationship between P. bivia to G. vaginalis is proposed, with ammonia flow as a mechanism to support this hypothesis.
作为细菌性阴道病发病机制正在进行的研究的一部分，对6株二路普雷沃菌和4株阴道加德纳菌进行了营养底物利用检测。向阴道限定培养基（VDM）中添加单一氨基酸对二路普雷沃菌的生长有促进作用，但对阴道加德纳菌没有作用。然而，肽显著促进了这两种菌的生长。二路普雷沃菌在VDM以及添加了氨基酸或蛋白胨的VDM中生长时伴随着净氨产生，而阴道加德纳菌在相同条件下生长则导致净氨利用。二路普雷沃菌在添加蛋白胨的VDM中生长产生的富含氨的上清液对阴道加德纳菌的生长有促进作用。然而，阴道加德纳菌在添加蛋白胨的VDM中生长产生的氨减少的上清液对二路普雷沃菌的生长有中性作用。提出了二路普雷沃菌与阴道加德纳菌之间的共生关系，并以氨流作为支持这一假说的机制。
Zotero 插件