高糖基化葡萄糖淀粉酶在亲本宿主泡盛曲霉变种川内曲霉中的表达及功能分析。
Expression and functional analysis of a hyperglycosylated glucoamylase in a parental host, Aspergillus awamori var. kawachi.
作者信息
Goto M, Ekino K, Furukawa K
机构信息
Department of Agricultural Chemistry, Kyushu University, Fukuoka, Japan.
出版信息
Appl Environ Microbiol. 1997 Jul;63(7):2940-3. doi: 10.1128/aem.63.7.2940-2943.1997.
Abstract
A modified glucoamylase gene (glaA) with an extra Thr- and Ser-rich Gp-I domain (T. Semimaru, M. Goto, K. Furukawa, and S. Hayashida, Appl. Environ. Microbiol. 61:2885-2890, 1995) was introduced into a mutant parental host, Aspergillus awamori var. kawachi, in which the original glaA gene had been completely deleted and replaced with the hygromycin phosphotransferase gene. The modified glaA was successfully expressed and secreted. The modified glucoamylase possessed higher digestibility of raw corn starch and higher stabilities in response to heat and extreme pH.
摘要
一个带有额外富含苏氨酸和丝氨酸的Gp-I结构域的修饰型糖化酶基因（glaA）（T. Semimaru、M. Goto、K. Furukawa和S. Hayashida，《应用与环境微生物学》61:2885 - 2890，1995年）被导入一个突变的亲本宿主泡盛曲霉变种河内曲霉中，在该宿主中原始的glaA基因已被完全删除并被潮霉素磷酸转移酶基因取代。修饰后的glaA成功表达并分泌。修饰后的糖化酶对生玉米淀粉具有更高的消化率，并且在热和极端pH条件下具有更高的稳定性。
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