Fiser A, Láníková A
University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
Vet Med (Praha). 1997 Jun;42(6):171-7.
Air and contact indicator microflora was monitored for a year during 17 outpatients' examinations at indicated sites of the balance loading area, examination tables and manipulation tables in an outpatients' consulting room partitioned into two sections (I, II) and in two waiting rooms (I, II) of an internal clinic of the Faculty of Veterinary Medicine at Brno. The consulting room and both waiting rooms were after reconstruction. Ambience monitoring by a sedimentation method demonstrated the lower microbial contamination of waiting room II and outpatients' consulting room II, which were reserved for vaccination of healthy animals. The ambience in waiting room I and outpatients' consulting room I, where diseased animals were examined, showed the higher values microbial contamination. The indicators of microbial contamination under observation involved total microbial counts on meat-peptone agar (MPA), counts of hemolytic (H+) and nonhemolytic (H+) micrococci on blood agar with 10% NaCl, counts of lactose positive (L+) and lactose negative (L-) microbes on Endo agar and of molds and other microbes on Czapek-Dox agar. Frequent occurrence of representatives of the genus Aspergillus and Penicillium out of 42 genera and species of identified molds was indicated in the ambience of the waiting rooms and outpatients' consulting rooms. Examination of the indicated sites of the balance loading area in waiting room I and of examination and manipulation tables in outpatients' consulting rooms by smear and impress methods demonstrated the lowest contamination on the surface of examination tables. Microbial contamination of the balance loading area was however highest, and the identified values indicate a risk of the permanent contact of animal patients with the increased microbe concentrations during their weighing. The balance location in the waiting room is not convenient under the present schedule of cleaning and cleansing. In the Discussion section, an overall decrease in the ambience microbial contamination in waiting rooms and outpatients' consulting room under observation in building no. 9 after reconstruction is shown in comparison with the results of preceding monitoring of the waiting room and outpatients' consulting room in building no. 6 under provisional conditions.
在布尔诺兽医学院内科诊所的一个分为两个区域(I、II)的门诊咨询室以及两个候诊室(I、II)中,对17名门诊患者在平衡称重区、检查台和操作台上指定部位进行检查期间,对空气和接触指示微生物群落进行了为期一年的监测。咨询室和两个候诊室均已重建。通过沉降法进行的环境监测表明,候诊室II和门诊咨询室II的微生物污染较低,这两个区域是为健康动物接种疫苗预留的。检查患病动物的候诊室I和门诊咨询室I的环境显示,微生物污染值较高。观察的微生物污染指标包括肉蛋白胨琼脂(MPA)上的总微生物计数;含10%氯化钠的血琼脂上溶血(H+)和非溶血(H+)微球菌的计数;伊红美蓝琼脂上乳糖阳性(L+)和乳糖阴性(L-)微生物的计数以及察氏琼脂上霉菌和其他微生物的计数。在候诊室和门诊咨询室的环境中,在所鉴定的42个霉菌属和种中,曲霉属和青霉属代表频繁出现。通过涂片和压印法对候诊室I中平衡称重区的指定部位以及门诊咨询室中的检查台和操作台进行检查,结果表明检查台表面的污染最低。然而,平衡称重区的微生物污染最高,所确定的值表明动物患者在称重过程中与微生物浓度增加的区域长期接触存在风险。按照目前的清洁和消毒时间表,候诊室中的天平位置不方便。在讨论部分,与之前在临时条件下对6号楼候诊室和门诊咨询室进行监测的结果相比,展示了重建后9号楼中观察到的候诊室和门诊咨询室环境微生物污染的总体下降情况。