人血中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性：性别、年龄和吸烟的影响。

Superoxide dismutase, catalase and glutathione peroxidase activities in human blood: influence of sex, age and cigarette smoking.

作者信息

Bolzán A D, Bianchi M S, Bianchi N O

机构信息

Instituto Multidisciplinario de Biología Celular (IMBICE), La Plata, Argentina.

出版信息

Clin Biochem. 1997 Aug;30(6):449-54. doi: 10.1016/s0009-9120(97)00047-7.

DOI:10.1016/s0009-9120(97)00047-7

PMID:9316738

Abstract

OBJECTIVE

To obtain reference ranges for each of the main antioxidant enzymes (AOE) and analyze the influence of sex, age, and cigarette smoking on AOE activity in human blood.

DESIGN AND METHODS

We investigated superoxide dismutase (SOD), catalase (CAT), and seleno-dependent glutathione peroxidase (GSH-Px) activities in the whole blood from 103 healthy subjects, from 18-67 years old (51 males and 52 females).

RESULTS

We found a large and highly significant interindividual variability in the activity of all the AOE studied (p < 0.001). The interindividual coefficients of variation were 13.5% for SOD, 21.0% for CAT, and 36.2% for GSH-Px, indicating that GSH-Px exhibits the highest interindividual variability. Females showed higher SOD (p < 0.001) and CAT (p < 0.001) activities but lower GSH-Px (p < 0.05) activity than males. We found a significant effect of age on SOD activity (p < 0.001), showing that in human blood it decreases with age and that this decrease is not linear, beginning at 28 years of age. We also observed a linear effect of age on GSH-Px activity indicating that the activity of this enzyme increases with age (p < 0.01). No effect of age on CAT activity was observed (p > 0.05). AOE activity in smokers was found not to be significantly different from that observed in non-smokers (p > 0.05) except in the case of CAT activity in females, which was found to be lower in smokers than in non-smokers (p < 0.05). In addition, we determined reference ranges for the activity of each antioxidant enzyme studied.

CONCLUSIONS

Our results confirm that AOE activity in human blood exhibits a wide interindividual variability and suggest that this variability may be ascribed, at least in part, to the sex and age of the individuals. Moreover, our results suggest that cigarette smoking does not influence AOE activity in human blood. Accordingly, it is suggested that for clinical purposes it may be necessary to consider the sex and age of the subjects involved in the study.

摘要

目的

获取每种主要抗氧化酶（AOE）的参考范围，并分析性别、年龄和吸烟对人体血液中AOE活性的影响。

设计与方法

我们调查了103名年龄在18至67岁之间的健康受试者（51名男性和52名女性）全血中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和硒依赖性谷胱甘肽过氧化物酶（GSH-Px）的活性。

结果

我们发现所研究的所有AOE活性存在很大且高度显著的个体间差异（p < 0.001）。SOD的个体间变异系数为13.5%，CAT为21.0%，GSH-Px为36.2%，表明GSH-Px表现出最高的个体间变异性。女性的SOD（p < 0.001）和CAT（p < 0.001）活性高于男性，但GSH-Px活性低于男性（p < 0.05）。我们发现年龄对SOD活性有显著影响（p < 0.001），表明在人体血液中SOD活性随年龄降低，且这种降低不是线性的，从28岁开始。我们还观察到年龄对GSH-Px活性有线性影响，表明该酶的活性随年龄增加（p < 0.01）。未观察到年龄对CAT活性有影响（p > 0.05）。除女性CAT活性外，吸烟者的AOE活性与非吸烟者相比无显著差异（p > 0.05），女性吸烟者的CAT活性低于非吸烟者（p < 0.05）。此外，我们确定了所研究的每种抗氧化酶活性的参考范围。