用于检测溃疡分枝杆菌的免疫磁珠分离法和聚合酶链反应
Immunomagnetic separation and PCR for detection of Mycobacterium ulcerans.
作者信息
Roberts B, Hirst R
机构信息
Department of Microbiology and Immunology, James Cook University of North Queensland, Townsville, Australia.
出版信息
J Clin Microbiol. 1997 Oct;35(10):2709-11. doi: 10.1128/jcm.35.10.2709-2711.1997.
Abstract
We have developed a technique based on the use of monodisperse magnetic beads to isolate Mycobacterium ulcerans from heterogenous mixtures, prior to PCR amplification. Using this method, we were able to detect M. ulcerans in water samples taken from Phillip Island, Australia, the site of several outbreaks of M. ulcerans disease in recent times.
摘要
我们开发了一种基于使用单分散磁珠的技术，用于在PCR扩增之前从异质混合物中分离溃疡分枝杆菌。使用这种方法，我们能够在从澳大利亚菲利普岛采集的水样中检测到溃疡分枝杆菌，该岛是近年来几次溃疡分枝杆菌病疫情的发生地。
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本文引用的文献
1
Detection of Clostridium difficile enterotoxin gene in clinical specimens by the polymerase chain reaction.
J Med Microbiol. 1993 May;38(5):384-7. doi: 10.1099/00222615-38-5-384.
2
Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens.
J Clin Microbiol. 1993 Jan;31(1):31-8. doi: 10.1128/jcm.31.1.31-38.1993.
3
Mycobacterium ulcerans infection on Phillip Island, Victoria.
Med J Aust. 1994 Feb 7;160(3):160.
4
Detection of pathogenic Yersinia enterocolitica in foods and water by immunomagnetic separation, nested polymerase chain reactions, and colorimetric detection of amplified DNA.
Appl Environ Microbiol. 1993 Sep;59(9):2938-44. doi: 10.1128/aem.59.9.2938-2944.1993.
5
Magnetic separation techniques in diagnostic microbiology.
Clin Microbiol Rev. 1994 Jan;7(1):43-54. doi: 10.1128/CMR.7.1.43.
6
Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens.
J Clin Microbiol. 1995 Aug;33(8):2162-5. doi: 10.1128/jcm.33.8.2162-2165.1995.
7
Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions.
J Clin Microbiol. 1990 Jun;28(6):1300-7. doi: 10.1128/jcm.28.6.1300-1307.1990.
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