Caine W R, Sauer W C, Tamminga S, Verstegen M W, Schulze H
Wageningen Institute of Animal Sciences, Department of Animal Nutrition, Wageningen Agricultural University, The Netherlands.
J Anim Sci. 1997 Nov;75(11):2962-9. doi: 10.2527/1997.75112962x.
Apparent ileal digestibilities of amino acids were determined in pigs fed cornstarch-based diets with untreated or protease-treated soybean meal as the protein source. Sixteen pigs were fitted with a modified post valve T-cecum cannula on d 14, 15, and 16 after birth and then returned to their sows until d 20 when they were weaned. Twelve of the pigs were selected for the study, which was conducted according to a two-period balanced change-over design. Treatments consisted of soybean meal that was 1) untreated (SBM), 2) processed by incubation (1:2 wt/vol distilled water adjusted to pH 4.5, for 16 h at 50 degrees C; CI-SBM), 3) sprayed with protease (supplied at 1 microL/g of soybean meal; PS-SBM), and 4) processed by incubation, as for CI-SBM, with protease in the water at the rate of 1 microL/g of soybean meal (PI-SBM). Each period consisted of 5 d of adaptation to diets followed by three 8-h collection periods (total of 24 h) that alternated with 8-h periods in which digesta were not collected. Apparent CP digestibilities were similar (P > .05) at 70.4, 72.4, 65.2, and 70.3% for the SBM, CI-SBM, PS-SBM, and PI-SBM diets, respectively. Corresponding amino acid digestibilities were also similar (P > .05), ranging from 62.5, 67.5, 57.9, and 65.0% for alanine to 83.5, 83.4, 78.7, and 84.7% for arginine. Apparent digestibilities were less (P < .05) for Period 1 (on d 7 after weaning) than for Period 2 (on d 16 after weaning). In conclusion, protease treatment of soybean meal had no effect on ileal digestibilities of CP and amino acids in newly weaned pigs.
以玉米淀粉为基础日粮,分别用未处理或经蛋白酶处理的豆粕作为蛋白质来源,测定仔猪氨基酸的表观回肠消化率。16头仔猪在出生后第14、15和16天安装改良的回肠末端T型盲肠插管,然后放回母猪身边直至第20天断奶。选择其中12头仔猪进行研究,试验采用两阶段平衡转换设计。处理组包括:1)未处理的豆粕(SBM);2)经孵育处理的豆粕(1:2质量/体积蒸馏水,调至pH 4.5,50℃孵育16小时;CI-SBM);3)喷洒蛋白酶的豆粕(用量为1微升/克豆粕;PS-SBM);4)如CI-SBM那样经孵育处理,且水中蛋白酶用量为1微升/克豆粕的豆粕(PI-SBM)。每个阶段包括5天适应日粮期,随后是三个8小时的收集期(共24小时),期间穿插8小时不收集食糜的时间段。SBM、CI-SBM、PS-SBM和PI-SBM日粮的表观粗蛋白消化率分别为70.4%、72.4%、65.2%和70.3%,差异不显著(P>0.05)。相应的氨基酸消化率也相似(P>0.05),从丙氨酸的62.5%、67.5%、57.9%和65.0%到精氨酸的83.5%、83.4%、78.7%和84.7%。第1阶段(断奶后第7天)的表观消化率低于第2阶段(断奶后第16天)(P<0.05)。总之,蛋白酶处理豆粕对刚断奶仔猪的粗蛋白和氨基酸回肠消化率没有影响。