Beneke S, Meyer R, Bürkle A
Forschungsschwerpunkt Angewandte Tumorvirologie, Abt. Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Biochem Mol Biol Int. 1997 Nov;43(4):755-61. doi: 10.1080/15216549700204571.
Poly(ADP-ribose) polymerase (EC 2.4.2.30) is a nuclear enzyme which binds to DNA breaks and then catalyzes the covalent modification of acceptor proteins with poly(ADP-ribose). Poly(ADP-ribose) polymerase activity contributes to the recovery of proliferating cells from DNA damage and to the maintenance of genomic stability, which may be mediated by effects on chromatin structure, DNA base-excision repair and cell cycle regulation. We established the complete cDNA sequence of rat poly(ADP-ribose) polymerase by RT-PCR and direct sequencing of amplification products and compared it with that of other mammalian species. The amino acid sequence homology is strikingly high. The best conserved regions are the known functional modules of poly(ADP-ribose) polymerase.
聚（ADP-核糖）聚合酶（EC 2.4.2.30）是一种核酶，它与DNA断裂处结合，然后催化用聚（ADP-核糖）对受体蛋白进行共价修饰。聚（ADP-核糖）聚合酶活性有助于增殖细胞从DNA损伤中恢复，并有助于维持基因组稳定性，这可能是通过对染色质结构、DNA碱基切除修复和细胞周期调控的影响来介导的。我们通过逆转录聚合酶链反应（RT-PCR）和扩增产物的直接测序建立了大鼠聚（ADP-核糖）聚合酶的完整cDNA序列，并将其与其他哺乳动物物种的序列进行了比较。氨基酸序列同源性非常高。保守性最好的区域是聚（ADP-核糖）聚合酶已知的功能模块。
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