H1变体过量产生对基因表达的差异效应归因于中央球状结构域的差异。
Differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain.
作者信息
Brown D T, Gunjan A, Alexander B T, Sittman D B
机构信息
Department of Biochemistry, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216-4505, USA.
出版信息
Nucleic Acids Res. 1997 Dec 15;25(24):5003-9. doi: 10.1093/nar/25.24.5003.
Abstract
The in vivo overproduction of two mouse histone H1 variants in homologous mouse fibroblasts has opposite effects on gene expression. Overproduction of H1(0) results in repression of transcript levels of all polymerase II genes tested. In contrast, overproduction of H1c results in elevated levels of transcripts. We created a series of chimeric H1 genes in which the regions encoding the three structural domains common to this family of these proteins were systematically switched. Overexpression of these genes in vivo resulted in the accumulation of large amounts of the chimeric H1 in chromatin. Analysis of the effects of overproduction of these proteins revealed that the differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain.
摘要
在同源小鼠成纤维细胞中两种小鼠组蛋白H1变体的体内过量表达对基因表达具有相反的影响。H1(0)的过量表达导致所有测试的聚合酶II基因转录水平的抑制。相反，H1c的过量表达导致转录物水平升高。我们构建了一系列嵌合H1基因，其中编码该蛋白家族共有的三个结构域的区域被系统地交换。这些基因在体内的过表达导致大量嵌合H1在染色质中积累。对这些蛋白质过量表达的影响分析表明，H1变体过量表达对基因表达的差异效应是由于中央球状结构域的差异。
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