Jenssen R, Jenssen H L, Köhler H, Friemel H
Mod Probl Ophthalmol. 1976;16:259-64.
The MEM test by FIELD and CASPARY has been investigated for its importance in analyzing the cell-mediated immunity in ophthalmology. Sensitized lymphocytes of patients are incubated in vitro either with basic encephalitogenic protein for investigating malignoma or with various eye antigens for investigating chronic ocular diseases. The eye antigens have been fractionated by gel chromatography and identified by various immune sera. The sensitized lymphocytes are released in vitro by incubation with antigen a factor which reduces the electrophoretic mobility of guinea pig macrophages.
菲尔德(FIELD)和卡斯帕里(CASPARY)的微量淋巴细胞毒试验(MEM test)在眼科细胞介导免疫分析中的重要性已得到研究。患者的致敏淋巴细胞在体外与碱性致脑炎蛋白一起孵育以研究恶性肿瘤,或与各种眼抗原一起孵育以研究慢性眼部疾病。眼抗原已通过凝胶色谱法进行分离,并通过各种免疫血清进行鉴定。致敏淋巴细胞通过与抗原一起孵育在体外释放,抗原是一种能降低豚鼠巨噬细胞电泳迁移率的因子。