禽致病性大肠杆菌菌株的黏附菌毛

Adherence pili of pathogenic strains of avian Escherichia coli.

作者信息

Vidotto M C, Navarro H R, Gaziri L C

机构信息

Universidade Estadual de Londrina, Campus Universitário, Londrina, Brasil.

出版信息

Vet Microbiol. 1997 Dec;59(1):79-87. doi: 10.1016/s0378-1135(97)00124-7.

DOI:10.1016/s0378-1135(97)00124-7

PMID:9460199

Abstract

Fifty strains of Escherichia coli isolated from colisepticemic chickens in Londrina, Brazil, were examined for presence of gene sequences for pil and pap, hemagglutination, and adherence to chicken tracheal cells. Forty-one strains were pil+ and 22 of these showed mannose sensitive (MS) hemagglutination (MSHA) of guinea-pig erythrocytes, indicating that they possessed only type 1 pili. Seven strains were pap+ and 6 of these caused mannose resistant (MR) hemagglutination (MRHA) of human erythrocytes. Twenty-four strains (17 of which caused MSHA) showed MS-adherence to chicken tracheal cells and the remaining 26 showed MR-adherence. The former typically adhered to the mucus layer whereas the latter usually adhered to the mucosal epithelium. It is concluded that MS adherence to chicken tracheal cells is correlated with expression of type 1 fimbriae and that MR-adherence to chicken tracheal cells cannot always be attributed to P pili.

摘要

对从巴西隆德里纳患败血症的鸡中分离出的50株大肠杆菌进行了检测，以确定其是否存在菌毛（pil）和菌毛相关蛋白（pap）的基因序列、血细胞凝集情况以及对鸡气管细胞的黏附性。41株菌为pil +，其中22株对豚鼠红细胞表现出甘露糖敏感（MS）血细胞凝集（MSHA），表明它们仅具有1型菌毛。7株菌为pap +，其中6株可使人红细胞发生甘露糖抗性（MR）血细胞凝集（MRHA）。24株菌（其中17株引起MSHA）对鸡气管细胞表现出MS黏附性，其余26株表现出MR黏附性。前者通常黏附于黏液层，而后者通常黏附于黏膜上皮。得出的结论是，对鸡气管细胞的MS黏附与1型菌毛的表达相关，并且对鸡气管细胞的MR黏附不能总是归因于P菌毛。