诺卡氏菌溶原菌交配中诺卡氏菌噬菌体phi EC的遗传。
Inheritance of nocardiophage phi EC in matings of nocardial lysogens.
作者信息
Brownell G H, Adams J N
出版信息
J Bacteriol. 1976 Jun;126(3):1104-7. doi: 10.1128/jb.126.3.1104-1107.1976.
Lysogens of Nocardia erythropolis were mated with nonlysogenic strains to study the inheritance of the phi EC prophage. Crosses between lysogenic strains of the Mat-Ce mating type and nonlysogenic Mat-cE strains produced Mat-cE lysogens at a recovery rate of 17%, whereas recombination frequencies between chromosomal traits were about 2.3 x 10(-5). Crosses of lysogenic Mat-cE mating types with nonlysogenic Mat-Ce produced Mat-Ce lysogens at a recovery rate of 19%, whereas recombinants for chromosomal traits were recovered at only 1.8 x 10(-5). Crosses of homologous mating types, lysogenic Mat-Ce with nonlysogenic Mat-Ce or lysogenic Mat-cE with nonlysogenic Mat-cE, failed to transfer the prophage. It was concluded that the phi EC prophage exists as a plasmid and can be transferred at high frequencies with patterns of transfer controlled like typical nocardial fertility. Evidence that the prophage may also exist as an integrated element was observed from recombination analyses.
将红斑诺卡氏菌的溶源菌与非溶源菌进行交配，以研究φEC原噬菌体的遗传情况。Mat-Ce交配型的溶源菌与非溶源的Mat-cE菌株之间的杂交产生Mat-cE溶源菌，回收率为17%，而染色体性状之间的重组频率约为2.3×10⁻⁵。溶源的Mat-cE交配型与非溶源的Mat-Ce杂交产生Mat-Ce溶源菌，回收率为19%，而染色体性状的重组体仅以1.8×10⁻⁵的频率回收。同源交配型之间的杂交，即溶源的Mat-Ce与非溶源的Mat-Ce或溶源的Mat-cE与非溶源的Mat-cE，未能转移原噬菌体。得出的结论是，φEC原噬菌体以质粒形式存在，并且可以以类似于典型诺卡氏菌育性的转移模式进行高频转移。从重组分析中观察到原噬菌体也可能以整合元件形式存在的证据。
Zotero 插件