利用聚合酶链反应技术对人流产组织中的细小病毒B19、巨细胞病毒和人乳头瘤病毒进行评估。

Evaluation of Parvo B19, CMV and HPV viruses in human aborted material using the polymerase chain reaction technique.

作者信息

Sifakis S, Ergazaki M, Sourvinos G, Koffa M, Koumantakis E, Spandidos D A

机构信息

Department of Obstetrics and Gynecology, University Hospital, Heraklion, Crete, Greece.

出版信息

Eur J Obstet Gynecol Reprod Biol. 1998 Feb;76(2):169-73. doi: 10.1016/s0301-2115(97)00208-x.

DOI:10.1016/s0301-2115(97)00208-x

PMID:9481569

Abstract

OBJECTIVE

To investigate the role of human parvovirus B19 (Parvo B19), cytomegalovirus (CMV) and human papilloma virus (HPV) viruses in the aetiopathogenesis of spontaneous abortions.

STUDY DESIGN

Abortion material from 102 cases of women with spontaneous abortions were analysed for the presence of Parvo B19, CMV and HPV DNA using the polymerase chain reaction (PCR) technique. Serological assays were used for the detection of specific IgM and IgG antibodies against Parvo B19 virus and CMV in the maternal sera.

RESULTS

Parvo B19 virus genome was detected in two cases of spontaneous abortion, by PCR amplification, while CMV and HPV genomes were not observed. Serological markers were indicative for Parvo B19 virus and CMV infection in ten and four cases, respectively.

CONCLUSIONS

PCR is a useful method for investigating the viral contribution to the aetiopathogenesis of spontaneous abortions and for detecting the viral genome in the abortion material. This study of 102 cases of spontaneous abortion does not implicate CMV and HPV in the aetiopathogenesis of spontaneous abortion, although it indicates a possible abortional role for Parvo B19 virus.

摘要