Kähler C M, Herold M, Kaufmann G, Pischel A B, Schratzberger P, Reinisch N, Gruber B, Bellmann R, Dunzendorfer S, Kieselbach G, Wiedermann C J
Department of Internal Medicine, Faculty of Medicine, University of Innsbruck, Austria.
Eur J Pharmacol. 1998 Jan 2;341(1):111-7. doi: 10.1016/s0014-2999(97)01466-0.
Proliferative vitreoretinopathy is a severe ocular disorder characterized by unwanted proliferation of cells and excessive production of fibrous tissue, which leads to the formation of cellular membranes on the surface of the retina and in the vitreous. Proliferative vitreoretinopathy is the most common cause of failure in retinal reattachment surgery, approximately occurring in one out of ten operated eyes. Proliferation of retinal pigment epithelial cells and fibroblasts is a cornerstone in the pathogenesis of proliferative vitreoretinopathy. An in vitro-proliferation assay showed previously that intraocular fluid from patients with proliferative vitreoretinopathy is potently effective in stimulating proliferation of human fibroblasts. Here we show that exposure of human fibroblasts to vitreous fluids from patients with proliferative vitreoretinopathy causes a rapid and sustained increase in arachidonic acid metabolite release as measured by competitive enzyme-immunoassay. The findings implicate prostaglandin E2 as a contributor to enhanced intraocular fibrosis in proliferative vitreoretinopathy. As prostaglandin E2 is a mediator of continuous aqueous-blood retinal barrier breakdown in this severe disease, cycclooxygenase inhibitors such as acetylsalicylic acid, which was successfully used in this study for blocking the effect of intraocular fluid, may be useful agents in targeting the progression of intraocular fibrosis.
增殖性玻璃体视网膜病变是一种严重的眼部疾病，其特征是细胞异常增殖和纤维组织过度产生，导致视网膜表面和玻璃体内形成细胞膜。增殖性玻璃体视网膜病变是视网膜复位手术失败的最常见原因，大约每十只手术眼中就有一只会发生。视网膜色素上皮细胞和成纤维细胞的增殖是增殖性玻璃体视网膜病变发病机制的基石。先前的一项体外增殖试验表明，增殖性玻璃体视网膜病变患者的眼内液在刺激人成纤维细胞增殖方面具有显著效果。在此我们表明，将人成纤维细胞暴露于增殖性玻璃体视网膜病变患者的玻璃体液中，通过竞争性酶免疫测定法测量，会导致花生四烯酸代谢物释放迅速且持续增加。这些发现表明前列腺素E2是增殖性玻璃体视网膜病变中眼内纤维化增强的一个促成因素。由于前列腺素E2是这种严重疾病中血-视网膜屏障持续破坏的介质，环氧化酶抑制剂（如本研究中成功用于阻断眼内液作用的阿司匹林）可能是针对眼内纤维化进展的有用药物。
