犬金属硫蛋白-III基因的分子克隆与表达

Molecular cloning and expression of the canine metallothionein-III gene.

作者信息

Kojima S, Shimada A, Kodan A, Kobayashi K, Morita T, Yamano Y, Umemura T

机构信息

Department of Veterinary Pathology, Tottori University, Japan.

出版信息

Can J Vet Res. 1998 Apr;62(2):148-51.

PMID:9553716

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1189463/

Abstract

We have isolated and determined the complete nucleotide sequence of canine metallothionein-III (MT-III) cDNA. The predicted amino acid sequence of the canine MT-III showed a high homology (93%, 87% identity) to that of human and mouse MT-III. The canine MT-III had 2 insertions relative to known mammalian MT-I and MT-II: a threonine after the 4th amino acid and a block of 6 amino acids near the carboxyl terminus. Expression of the canine MT-III mRNA was found exclusively in the central nervous system, where neurons in the olfactory bulb, hippocampus and cerebral cortex showed predominant signals.

摘要

我们已经分离并测定了犬金属硫蛋白-III（MT-III）cDNA的完整核苷酸序列。犬MT-III的预测氨基酸序列与人类和小鼠的MT-III具有高度同源性（93%，87%的同一性）。相对于已知的哺乳动物MT-I和MT-II，犬MT-III有2个插入：第4个氨基酸后有一个苏氨酸，羧基末端附近有一个6个氨基酸的片段。犬MT-III mRNA的表达仅在中枢神经系统中发现，嗅球、海马体和大脑皮层的神经元显示出主要信号。