黑腹栉足蛛毒素可阻断墨西哥金背蜘蛛毒素诱导的突触体钙内流。

Phoneutria nigriventer toxins block tityustoxin-induced calcium influx in synaptosomes.

作者信息

Miranda D M, Romano-Silva M A, Kalapothakis E, Diniz C R, Cordeiro M N, Santos T M, Prado M A, Gomez M V

机构信息

Departmento de Farmacologia, ICB-UFMG, Belo Horizonte-MG, Brasil.

出版信息

Neuroreport. 1998 May 11;9(7):1371-3. doi: 10.1097/00001756-199805110-00022.

DOI:10.1097/00001756-199805110-00022

PMID:9631431

Abstract

Neurotoxins can help the understanding of mechanisms involved in neurotransmission. We here report that two neurotoxin isoforms, Tx3-3 and Tx3-4 obtained from the venom of the spider Phoneutria nigriventer inhibited the 45Ca2+ influx in rat cortical synaptosomes induced by the scorpion venom tityustoxin. The IC50 for Tx3-3 and Tx3-4 were 0.32 and 7.9 nM, respectively. The neurotoxins Tx3-3 and Tx3-4 are very effective in inhibiting 45Ca2+ influx and they should be useful in studies involving Ca(2+)-dependent processes.

摘要

神经毒素有助于理解神经传递所涉及的机制。我们在此报告，从黑腹捕鸟蛛毒液中获得的两种神经毒素亚型Tx3-3和Tx3-4，可抑制由蝎毒tityustoxin诱导的大鼠皮质突触体中的45Ca2+内流。Tx3-3和Tx3-4的半数抑制浓度（IC50）分别为0.32和7.9纳摩尔。神经毒素Tx3-3和Tx3-4在抑制45Ca2+内流方面非常有效，它们应有助于涉及钙（2+）依赖性过程的研究。