猫踝关节肌肉中纤维组成与自发活动每日时长的关系。

Relation between fibre composition and daily duration of spontaneous activity in ankle muscles of the cat.

作者信息

Kernell D, Hensbergen E, Lind A, Eerbeek O

机构信息

Department of Neurophysiology, University of Amsterdam, The Netherlands.

出版信息

Arch Ital Biol. 1998 Jul;136(3):191-203.

PMID:9645309

Abstract

UNLABELLED

This study concerns the relation between use and fibre type composition among limb muscles. The histochemical properties were investigated for ankle muscles from cats that had previously been studied in 24 hr electromyographic (EMG) recordings of daily spontaneous activity. We then reported average daily "duty times" (i.e. the percentage of total sampling time filled with EMG activity) of 1.9% for extensor digitorum longus (EDL), 2.1 and 4.0% for anterior and posterior sites of tibialis anterior (TA), 6.6 and 9.5% for anterior and posterior sites of peroneus longus (PL), and 13.9% for soleus (SOL). In the present experiments, muscles from which these data had been obtained were sectioned in a cryostat and stained for myofibrillar ATPase. Fibres were classified as type I (presumably slow) or II (presumably fast), the latter fibres being further categorized as IIA, IIB and a minor portion of transitional IIAB fibres. As expected, SOL was 100% type I. Among the muscles of mixed fibre-type composition ("mixed muscles"), a statistically significant difference in the mean percentages of type I fibres was found between TA or EDL (2.9-6.0%) vs. PL (11.8-14.6%). For TA the percentage of type I fibres was higher in posterior (deep) than in anterior (superficial) sampling regions; for PL no clear antero-posterior difference was found. A significant correlation was obtained between the percentage of type I fibres and the total duration of daily activity recorded from corresponding mixed muscle sites (5 different recording sites in 4 cats, totally 15 cases of successfully combined physiological and histochemical measurements, r = 0.76, P < 0.001). Similarly, within TA the total duration of daily activity was higher for sites with high (posteriorly) than for those with low (anteriorly) percentages of type I fibres.

IN CONCLUSION

a "coarse-grain" relationship was found between fibre type composition and the duration of daily activity among mixed muscles. Possible mechanisms underlying this relationship are discussed.

摘要

未标注

本研究关注肢体肌肉的使用情况与纤维类型组成之间的关系。对先前在每日自发活动的24小时肌电图（EMG）记录中研究过的猫的踝关节肌肉的组织化学特性进行了研究。我们随后报告了趾长伸肌（EDL）的平均每日“工作时间”（即充满EMG活动的总采样时间的百分比）为1.9%，胫骨前肌（TA）前侧和后侧部位分别为2.1%和4.0%，腓骨长肌（PL）前侧和后侧部位分别为6.6%和9.5%，比目鱼肌（SOL）为13.9%。在本实验中，获取了这些数据的肌肉在低温恒温器中切片，并进行肌原纤维ATP酶染色。纤维被分类为I型（可能是慢肌纤维）或II型（可能是快肌纤维），后者进一步分为IIA、IIB和一小部分过渡性IIAB纤维。正如预期的那样，SOL为100%的I型纤维。在混合纤维类型组成的肌肉（“混合肌肉”）中，TA或EDL（2.9 - 6.0%）与PL（11.8 - 14.6%）之间I型纤维的平均百分比存在统计学上的显著差异。对于TA，I型纤维的百分比在后部（深层）采样区域高于前部（浅层）采样区域；对于PL，未发现明显的前后差异。I型纤维的百分比与从相应混合肌肉部位记录的每日活动总持续时间之间存在显著相关性（4只猫中的5个不同记录部位，总共15例成功结合生理和组织化学测量的案例，r = 0.76，P < 0.001）。同样，在TA内，I型纤维百分比高（后部）的部位的每日活动总持续时间高于I型纤维百分比低（前部）的部位。