F344大鼠中由氧化偶氮甲烷诱导的实验性结直肠癌发生过程中Apc基因的突变
Mutations of the Apc gene in experimental colorectal carcinogenesis induced by azoxymethane in F344 rats.
作者信息
De Filippo C, Caderni G, Bazzicalupo M, Briani C, Giannini A, Fazi M, Dolara P
机构信息
Department of Pharmacology, University of Florence, Italy.
出版信息
Br J Cancer. 1998 Jun;77(12):2148-51. doi: 10.1038/bjc.1998.359.
Abstract
We investigated in the rat the role of the Apc gene, which is mutated in familial adenomatous polyposis and sporadic colon cancer in the process leading from normal colonic mucosa to aberrant crypt foci (ACF) and finally to adenomas and adenocarcinomas. We analysed mutations in exon 15 of the rat Apc gene using in vitro synthesized protein assay in 66 ACF and in 28 colon tumours induced by azoxymethane. No Apc mutations were found in ACF, whereas five mutations were found in the tumours. The data suggest that mutations of the Apc gene are associated with the transition from ACF to adenoma and adenocarcinoma and not from normal mucosa to ACF.
摘要
我们在大鼠中研究了Apc基因的作用，该基因在家族性腺瘤性息肉病和散发性结肠癌中发生突变，在从正常结肠黏膜发展到异常隐窝灶（ACF）并最终发展为腺瘤和腺癌的过程中发挥作用。我们使用体外合成蛋白分析法分析了66个ACF和28个由氧化偶氮甲烷诱导的结肠肿瘤中大鼠Apc基因第15外显子的突变情况。在ACF中未发现Apc突变，而在肿瘤中发现了5个突变。数据表明，Apc基因的突变与从ACF向腺瘤和腺癌的转变有关，而不是与从正常黏膜向ACF的转变有关。
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