Bachmann M, Deister H, Pautz A, Laubinger J, Schmitz M, Falke D, Podlech J, Grölz D
Institut für Physiologische Chemie, Johannes-Gutenberg Universität, Mainz, Germany.
Clin Exp Immunol. 1998 Jun;112(3):482-9. doi: 10.1046/j.1365-2249.1998.00605.x.
Permanently transfected mouse cell lines which expressed different levels of the human autoantigen La/SS-B were infected with different strains of herpes simplex virus type 1, including the strains ANG, HSZP, 17syn+ and HFEM. During infection the localization of the human La protein was followed using an anti-La MoAb, which recognized only the human La protein but did not cross-react with either the endogenous mouse La protein or any viral encoded protein. After infection La protein was transported from the nucleus to the cytoplasm. The time course of translocation was dependent on the amount of human La protein expressed in the respective cell line. Moreover, acceleration of viral replication was dependent on the level of expression of human La protein, suggesting that La protein is a cellular factor that facilitates virus replication.
用不同株单纯疱疹病毒1型(包括ANG、HSZP、17syn +和HFEM株)感染稳定转染了表达不同水平人自身抗原La/SS - B的小鼠细胞系。在感染期间,使用仅识别人La蛋白而不与内源性小鼠La蛋白或任何病毒编码蛋白发生交叉反应的抗La单克隆抗体追踪人La蛋白的定位。感染后,La蛋白从细胞核转运至细胞质。转运的时间进程取决于各细胞系中人La蛋白的表达量。此外,病毒复制的加速取决于人La蛋白的表达水平,这表明La蛋白是一种促进病毒复制的细胞因子。