快速检测鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌的分子方法。
Molecular procedure for rapid detection of Burkholderia mallei and Burkholderia pseudomallei.
作者信息
Bauernfeind A, Roller C, Meyer D, Jungwirth R, Schneider I
机构信息
Max von Pettenkofer Institut, D-80336 Munich, Germany.
出版信息
J Clin Microbiol. 1998 Sep;36(9):2737-41. doi: 10.1128/JCM.36.9.2737-2741.1998.
Abstract
A PCR procedure for the discrimination of Burkholderia mallei and Burkholderia pseudomallei was developed. It is based on the nucleotide difference T 2143 C (T versus C at position 2143) between B. mallei and B. pseudomallei detected in the 23S rDNA sequences. In comparison with conventional methods the procedure allows more rapid identification at reduced risk for infection of laboratory personnel.
摘要
开发了一种用于鉴别鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌的聚合酶链反应（PCR）方法。该方法基于在23S核糖体DNA（rDNA）序列中检测到的鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌之间的核苷酸差异T2143C（第2143位的T与C）。与传统方法相比，该方法能够更快速地进行鉴定，同时降低实验室人员感染的风险。
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