将触须比例法与聚合酶链式反应（PCR）相比较，以区分来自西非几内亚比绍的冈比亚按蚊指名亚种和梅拉斯按蚊。

The palpal ratio method compared with PCR to distinguish between Anopheles gambiae s.s. and A. melas from Guinea Bissau, West Africa.

作者信息

Pålsson K, Pinto J, do Rosario V E, Jaenson T G

机构信息

Department of Zoology, Uppsala University, Sweden.

出版信息

Acta Trop. 1998 Jun 15;70(1):101-7. doi: 10.1016/s0001-706x(98)00017-5.

DOI:10.1016/s0001-706x(98)00017-5

PMID:9707368

Abstract

We compared the palpal ratio method with the polymerase chain reaction (PCR) to distinguish between Anopheles gambiae s.s. and A. melas. At the end of the rainy season of 1995, female mosquitoes were collected indoors in the Antula area of Bissau, Guinea Bissau. A subsample of 354 mosquitoes were identified first with PCR and then with the palpal ratio method (study A). Subsequently, another 195 mosquitoes were identified first with the palpal ratio method and then with PCR (study B). The highest percentage (100%, n = 16) of correctly identified A. melas was obtained at the palpal ratio cut-off point of 0.83. However, at this point 4.0% (14/347) and 11.3% (21/186) of the A. gambiae were erroneously identified as A. melas in study A and B, respectively. This suggests that the palpal ratio method is not sufficiently reliable to distinguish between A. gambiae and A. melas from the Bissau area.

摘要

我们将触须比例法与聚合酶链反应（PCR）进行比较，以区分冈比亚按蚊指名亚种和梅拉斯按蚊。1995年雨季结束时，在几内亚比绍比绍的安图拉地区室内采集雌蚊。首先用PCR对354只蚊子的子样本进行鉴定，然后用触须比例法鉴定（研究A）。随后，先用触须比例法对另外195只蚊子进行鉴定，再用PCR鉴定（研究B）。在触须比例临界点为0.83时，正确鉴定出的梅拉斯按蚊比例最高（100%，n = 16）。然而，在这一点上，研究A和研究B中分别有4.0%（14/347）和11.3%（2/186）的冈比亚按蚊被错误地鉴定为梅拉斯按蚊。这表明触须比例法在区分来自比绍地区的冈比亚按蚊和梅拉斯按蚊时不够可靠。