Tani K, Masuhara M, Welikala N, Yamaguchi N, Nasu M
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
J Appl Microbiol. 1998 May;84(5):859-64. doi: 10.1046/j.1365-2672.1998.00421.x.
The response of river water microbial communities to chemical compounds was monitored under laboratory conditions using aniline as a model. Bacteria were collected from unpolluted and polluted sites. Bacterial abundance (plate and total direct counting) and its relation to aniline biodegradation was examined. Colony hybridization with 16S rRNA oligonucleotide probes was used to study the changes in microbial community structure during biodegradation of aniline. The changes in bacterial abundance and community structure were related to biodegradation of aniline. Burkholderia-Pseudomonas (rRNA group III), an authentic Alcaligenes group became dominant despite the initial differences in the microbial communities, suggesting that these genera are the main aniline degraders in the aquatic environment.
在实验室条件下，以苯胺为模型监测河水微生物群落对化学化合物的反应。细菌取自未受污染和受污染的地点。检测了细菌丰度（平板计数和总直接计数）及其与苯胺生物降解的关系。使用16S rRNA寡核苷酸探针进行菌落杂交，以研究苯胺生物降解过程中微生物群落结构的变化。细菌丰度和群落结构的变化与苯胺的生物降解有关。伯克霍尔德氏菌-假单胞菌（rRNA第三组），一个正宗的产碱菌属，尽管微生物群落最初存在差异，但仍成为优势菌属，这表明这些属是水生环境中主要的苯胺降解菌。
