使用无氯溶剂测定尿液中粪便甾醇的新方法。
New method for determination of fecal sterols in urine using non-chlorinated solvents.
作者信息
Börjesson E, Sundin A, Leeming R, Torstensson L
机构信息
Swedish University of Agricultural Science, Department of Microbiology, Uppsala.
出版信息
J Chromatogr B Biomed Sci Appl. 1998 Aug 25;713(2):438-42. doi: 10.1016/s0378-4347(98)00197-2.
A new method has been developed to determine a number of sterols in urine using non-chlorinated solvents, namely methyl tert.-butyl ether and methanol (the MTB method). The method involves liquid-liquid extraction, saponification, reextraction, silylation and final identification and quantification by GC-FID. The sterols determined were coprostanol, epicoprostanol, cholesterol and dihydrocholesterol. 5Alpha-cholestane was used as internal standard. The limit of detection for the sterols in this experiment was 2 microg l(-1) urine. Recovery of coprostanol over the range 5-100 microg l(-1) urine by this method was between 80 and 92%.
已开发出一种新方法，使用非氯化溶剂（即甲基叔丁基醚和甲醇，MTB法）来测定尿液中的多种甾醇。该方法包括液液萃取、皂化、再萃取、硅烷化，最后通过气相色谱 - 火焰离子化检测器（GC - FID）进行鉴定和定量。所测定的甾醇为粪甾烷醇、表粪甾烷醇、胆固醇和二氢胆固醇。5α - 胆甾烷用作内标。本实验中甾醇的检测限为2μg l⁻¹尿液。用此方法在5 - 100μg l⁻¹尿液范围内粪甾烷醇的回收率在80%至92%之间。
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