前列腺上皮内瘤变（PIN）中的端粒酶活性、端粒长度和DNA倍性

Telomerase activity, telomere length, and DNA ploidy in prostatic intraepithelial neoplasia (PIN).

作者信息

Koeneman K S, Pan C X, Jin J K, Pyle J M, Flanigan R C, Shankey T V, Diaz M O

机构信息

Department of Urology, Loyola University Medical Center and Cardinal Bernardin Cancer Center, Maywood, Illinois, USA.

出版信息

J Urol. 1998 Oct;160(4):1533-9.

PMID:9751408

Abstract

PURPOSE

To investigate the relationship of telomerase activity, telomere length, and DNA ploidy in high grade prostatic intraepithelial neoplasia (PIN).

MATERIALS AND METHODS

Tissue samples were carefully microdissected to obtain adenocarcinoma or PIN-containing tissue free of cancer. Telomerase activity was measured using the PCR-based telomeric repeat amplification protocol (TRAP). Telomere length was estimated from Southern blots of telomere restriction fragments (TRFs). DNA ploidy of PIN and carcinoma was determined by image analysis of adjacent Feulgen stained tissue sections.

RESULTS

Telomerase activity was found in 4 of 25 samples (16%) of high grade PIN. All telomerase positive PIN foci had a diploid DNA content. Although 5 of 25 samples (25%) of high grade PIN foci analyzed were DNA aneuploid, none of these demonstrated telomerase activity. Telomerase positive foci of prostate carcinoma (69% of all cancer foci analyzed) displayed heterogeneity in TRF length, with a mean TRF length two kilobase pairs shorter than that of telomerase negative specimens.

CONCLUSIONS

Telomerase activity is present in a low percentage of high-grade PIN foci, which are diploid by DNA content measurements.

摘要

目的

研究高级别前列腺上皮内瘤变（PIN）中端粒酶活性、端粒长度和DNA倍体之间的关系。

材料与方法

仔细显微切割组织样本，以获取不含癌的腺癌或含PIN的组织。使用基于聚合酶链反应的端粒重复序列扩增法（TRAP）测量端粒酶活性。通过端粒限制片段（TRF）的Southern印迹估计端粒长度。通过对相邻福尔根染色组织切片的图像分析确定PIN和癌的DNA倍体。

结果

在25个高级别PIN样本中的4个（16%）中发现了端粒酶活性。所有端粒酶阳性的PIN病灶均具有二倍体DNA含量。尽管在分析的25个高级别PIN病灶样本中有5个（25%）为DNA非整倍体，但这些样本均未显示端粒酶活性。前列腺癌的端粒酶阳性病灶（占所有分析的癌病灶的69%）在TRF长度上表现出异质性，其平均TRF长度比端粒酶阴性标本短2千碱基对。