Odinot P T, Meis J F, Hoogkamp-Korstanje J A, Melchers W J
Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
J Clin Pathol. 1998 Jun;51(6):444-9. doi: 10.1136/jcp.51.6.444.
The sensitive detection of pathogenic Yersinia enterocolitica in paraffin embedded tissue sections by in situ hybridisation (ISH).
Y enterocolitica infected cell lines, rat spleens, and patient biopsy specimens were used to compare conventional ISH, immune fluorescence assay (IFA) detection, and catalysed reporter deposition (CARD) signal amplification ISH.
CARD-ISH was shown to be more sensitive then conventional ISH and had a comparable sensitivity to IFA. In contrast to IFA, CARD-ISH preserved good tissue morphology.
CARD-ISH appeared to be a fast and sensitive ISH method for detecting Y enterocolitica in routinely processed tissue sections. Application of this method allows the combination of routine detection and cellular localisation of the pathogen within the infected tissue.
通过原位杂交（ISH）对石蜡包埋组织切片中的致病性小肠结肠炎耶尔森菌进行灵敏检测。
使用小肠结肠炎耶尔森菌感染的细胞系、大鼠脾脏和患者活检标本，比较传统ISH、免疫荧光测定（IFA）检测和催化报告沉积（CARD）信号放大ISH。
CARD-ISH比传统ISH更灵敏，且与IFA具有相当的灵敏度。与IFA不同，CARD-ISH能保持良好的组织形态。
CARD-ISH似乎是一种用于在常规处理的组织切片中检测小肠结肠炎耶尔森菌的快速灵敏的ISH方法。该方法的应用能够将病原体在感染组织内的常规检测与细胞定位相结合。
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