艾滋病合并巨细胞病毒视网膜炎患者玻璃体液中巨细胞病毒（CMV）的糖蛋白B亚型分析

Glycoprotein B subtyping of cytomegalovirus (CMV) in the vitreous of patients with AIDS and CMV retinitis.

作者信息

Chern K C, Chandler D B, Martin D F, Kuppermann B D, Wolitz R A, Margolis T P

机构信息

Francis I. Proctor Foundation, UCSF Medical Center 94143-0944, USA.

出版信息

J Infect Dis. 1998 Oct;178(4):1149-53. doi: 10.1086/515672.

DOI:10.1086/515672

PMID:9806048

Abstract

A 550-bp region of the cytomegalovirus (CMV) glycoprotein B (gB) gene was amplified by polymerase chain reaction (PCR) from 141 vitreous specimens of 120 patients with AIDS and CMV retinitis from three different metropolitan centers. The distribution of gB subtypes I, II, III, and IV were 19%, 43%, 12%, and 21%, respectively, based on restriction enzyme digestion patterns of PCR-amplified DNA. Two patients had simultaneous infection with two different gB subtypes. The ratio of gB subtypes was similar among the three geographically distinct patient populations. Two of 14 patients with bilateral vitreous specimens had different viral subtypes in each eye. In addition, different gB subtypes were observed in 1 of 6 patients with serial specimens. The ratio of different gB subtypes in the vitreous of patients with CMV retinitis is similar to that previously reported in the peripheral blood of patients with advanced AIDS.

摘要

采用聚合酶链反应（PCR）从来自三个不同大城市中心的120例患有艾滋病和巨细胞病毒性视网膜炎患者的141份玻璃体标本中扩增出一段550碱基对的巨细胞病毒（CMV）糖蛋白B（gB）基因区域。根据PCR扩增DNA的限制性酶切图谱，gB亚型I、II、III和IV的分布分别为19%、43%、12%和21%。两名患者同时感染了两种不同的gB亚型。在三个地理上不同的患者群体中，gB亚型的比例相似。14例双侧玻璃体标本患者中有2例每只眼睛的病毒亚型不同。此外，在6例有系列标本的患者中有1例观察到不同的gB亚型。巨细胞病毒性视网膜炎患者玻璃体中不同gB亚型的比例与先前在晚期艾滋病患者外周血中报道的比例相似。