嵌合HIV-1/HIV-2包膜蛋白用于HIV感染免疫诊断的研究：利用HIV-1 env基因内的翻译起始位点在大肠杆菌中进行表达和纯化。

The use of a chimera HIV-1/HIV-2 envelope protein for immunodiagnosis of HIV infection: its expression and purification in E. coli by use of a translation initiation site within HIV-1 env gene.

作者信息

Han B, Meng L, Song X, Chen Q, Wang H, Ling S, Ma X

机构信息

Institute of Basic Medical Sciences, Beijing, P.R. China.

出版信息

Biochem Mol Biol Int. 1998 Oct;46(3):607-17. doi: 10.1080/15216549800204132.

DOI:10.1080/15216549800204132

PMID:9818100

Abstract

A chimera HIV-1/HIV-2 envelope sequence composed of multiple conserved immunodominant epitopes of HIV-1 envelope protein (HIV-1 IIIB: env482-518 + env548-675) and the HIV-2 gp36 immunodominant epitope (env592-603), was constructed and directly over-expressed in E. coli by using a prokaryotic translation initiation sequence contained within the gene of HIV-1 envelope. The recombinant product was purified and applied in antibody-screening assay. The purified chimera antigen reacted with all the thirty-eight HIV-1 positive serum samples, the two HIV-2 serum samples, and had no cross-reaction with all the eighty-eight normal healthy serum sample. The results indicated that this recombinant chimera HIV-1/HIV-2 envelope protein could be useful for diagnostic purposes of HIV infection.

摘要

构建了一种嵌合的HIV-1/HIV-2包膜序列，其由HIV-1包膜蛋白的多个保守免疫显性表位（HIV-1 IIIB：env482-518 + env548-675）和HIV-2 gp36免疫显性表位（env592-603）组成，并通过使用HIV-1包膜基因中包含的原核翻译起始序列在大肠杆菌中直接进行过表达。对重组产物进行纯化，并将其应用于抗体筛选试验。纯化后的嵌合抗原与所有38份HIV-1阳性血清样本、2份HIV-2血清样本发生反应，且与所有88份正常健康血清样本均无交叉反应。结果表明，这种重组嵌合HIV-1/HIV-2包膜蛋白可用于HIV感染的诊断。