脂质和质粒设计在基因传递至哺乳动物细胞中的应用。

Application of lipids and plasmid design for gene delivery to mammalian cells.

作者信息

Scherman D, Bessodes M, Cameron B, Herscovici J, Hofland H, Pitard B, Soubrier F, Wils P, Crouzet J

机构信息

UMR 133 CNRS/Rhône-Poulenc Rorer, Vitry-sur-Seine, France.

出版信息

Curr Opin Biotechnol. 1998 Oct;9(5):480-5. doi: 10.1016/s0958-1669(98)80033-5.

DOI:10.1016/s0958-1669(98)80033-5

PMID:9821276

Abstract

Cationic lipids are widely used for in vitro gene transfer due to their efficiency. The major challenges for the improvement of in vivo cationic lipid-mediated gene delivery reside in the design of more biocompatible lipoplexes mimicking viral-mediated gene delivery and in understanding the fate of the lipoplexes within the cells.

摘要

阳离子脂质因其高效性而被广泛用于体外基因转移。改善体内阳离子脂质介导的基因递送面临的主要挑战在于设计更具生物相容性的脂质体复合物以模拟病毒介导的基因递送，以及了解脂质体复合物在细胞内的命运。