用于通过抗体催化快速产生可调谐荧光的醛醇传感器。
Aldol sensors for the rapid generation of tunable fluorescence by antibody catalysis.
作者信息
List B, Barbas C F, Lerner R A
机构信息
The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15351-5. doi: 10.1073/pnas.95.26.15351.
Abstract
The synthesis of novel fluorogenic retro-aldol substrates for aldolase antibody 38C2 is described. These substrates are efficiently and specifically processed by antibody aldolases but not by natural cellular enzymes. Together, the fluorogenic substrates and antibody aldolases provide reporter gene systems that are compatible with living cells. The broad scope of the antibody aldolase allows for the processing of a range of substrates that can be designed to allow fluorescence monitoring at a variety of wavelengths. We also have developed the following concept in fluorescent protein tags. beta-Diketones bearing a fluorescent tag are bound covalently by the aldolase antibody and not other proteins. We anticipate that proteins fused with the antibody can be tagged specifically and covalently within living cells with fluorophores of virtually any color, thereby providing an alternative to green fluorescent protein fusions.
摘要
本文描述了用于醛缩酶抗体38C2的新型荧光逆转醛醇底物的合成。这些底物能被抗体醛缩酶高效且特异性地处理，但不能被天然细胞酶处理。荧光底物和抗体醛缩酶共同提供了与活细胞兼容的报告基因系统。抗体醛缩酶的广泛作用范围使得一系列底物能够被处理，这些底物可被设计用于在多种波长下进行荧光监测。我们还在荧光蛋白标签方面开发了以下概念。带有荧光标签的β-二酮通过醛缩酶抗体而非其他蛋白质共价结合。我们预计，与该抗体融合的蛋白质能够在活细胞内被几乎任何颜色的荧光团特异性且共价地标记，从而为绿色荧光蛋白融合提供一种替代方法。
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