在拟南芥中克隆出一种与ABI1和ABI2具有同源性的新型蛋白磷酸酶2C（PP2C）。
Molecular cloning in Arabidopsis thaliana of a new protein phosphatase 2C (PP2C) with homology to ABI1 and ABI2.
作者信息
Rodriguez P L, Leube M P, Grill E
机构信息
Instituto de Biologia Molecular y Celular de Plantas, Universidad Politécnica-CSIC, Valencia, Spain.
出版信息
Plant Mol Biol. 1998 Nov;38(5):879-83. doi: 10.1023/a:1006012218704.
We report the cloning of both the cDNA and the corresponding genomic sequence of a new PP2C from Arabidopsis thaliana, named AtP2C-HA (for homology to ABI1/ABI2). The AtP2C-HA cDNA contains an open reading frame of 1536 bp and encodes a putative protein of 511 amino acids with a predicted molecular mass of 55.7 kDa. The AtP2C-HA protein is composed of two domains, a C-terminal PP2C catalytic domain and a N-terminal extension of ca. 180 amino acid residues. The deduced amino acid sequence is 55% and 54% identical to ABI1 and ABI2, respectively. Comparison of the genomic structure of the ABI1, ABI2 and AtP2C-HA genes suggests that they belong to a multigene family. The expression of the AtP2C-HA gene is up-regulated by abscisic acid (ABA) treatment.
我们报道了从拟南芥中克隆出一种新的PP2C的cDNA及其相应的基因组序列，命名为AtP2C-HA（与ABI1/ABI2同源）。AtP2C-HA cDNA包含一个1536 bp的开放阅读框，编码一个推定的由511个氨基酸组成的蛋白质，预测分子量为55.7 kDa。AtP2C-HA蛋白由两个结构域组成，一个C端PP2C催化结构域和一个约180个氨基酸残基的N端延伸。推导的氨基酸序列与ABI1和ABI2的同一性分别为55%和54%。ABI1、ABI2和AtP2C-HA基因的基因组结构比较表明它们属于一个多基因家族。AtP2C-HA基因的表达受脱落酸（ABA）处理上调。
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