将大片段DNA插入序列遗传转移至枯草芽孢杆菌168基因组的指定位点。
Genetic transfer of large DNA inserts to designated loci of the Bacillus subtilis 168 genome.
作者信息
Itaya M
机构信息
Mitsubishi Kasei Institute of Life Sciences, Machida-shi, Tokyo 194-8511, Japan.
出版信息
J Bacteriol. 1999 Feb;181(3):1045-8. doi: 10.1128/JB.181.3.1045-1048.1999.
Abstract
It was found that contiguous DNA segments of up to 50 kb can be transferred between Bacillus subtilis genomes when a sufficient length of the flanking genomic region is provided for homologous recombination, although the efficiency of transfer was reduced as the insert size increased. Inserts were translocated to different loci, where appropriate integration sites were created.
摘要
研究发现，当为同源重组提供足够长度的侧翼基因组区域时，长达50 kb的相邻DNA片段可在枯草芽孢杆菌基因组之间转移，不过转移效率会随着插入片段大小的增加而降低。插入片段被转移到不同位点，并在这些位点创建了合适的整合位点。
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