通过R因子RP1的转导缩短分离出一种非可传递的抗生素抗性质粒。

Isolation of a nontransmissible antibiotic resistance plasmid by transductional shortening of R factor RP1.

作者信息

Shipley P L, Olsen R H

出版信息

J Bacteriol. 1975 Jul;123(1):20-7. doi: 10.1128/jb.123.1.20-27.1975.

DOI:10.1128/jb.123.1.20-27.1975

PMID:1095551

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235686/

Abstract

A plasmid segregant carrying tetracycline and carbenicillin resistance markers has been isolated from R factor RP1 by transductional shortening with phage P22. The new plasmid RP1-S2, which has a molecular weight of 23 times 10-6, has lost the transfer, phage sensitivity, and neomycin resistance functions of RP1. It combines readily with a W group plasmid, R388, to form a transmissible carbenicillin and trimethoprim resistance plasmid, RWP1.

摘要

通过用噬菌体P22进行转导缩短，从R因子RP1中分离出了携带四环素和羧苄青霉素抗性标记的质粒分离物。新的质粒RP1-S2分子量为23×10⁶，已丧失了RP1的转移、噬菌体敏感性和新霉素抗性功能。它能轻易地与W组质粒R388结合，形成可转移的羧苄青霉素和甲氧苄氨嘧啶抗性质粒RWP1。