The aim of this study is to determine the normal values of serum cholesterol, triglyceride, VLDL-C, LDL-C, and HDL-C to obtain the reference values for diagnostic tests in healthy children. The study group included 420 healthy children (209 male, 211 female) ranging from 0 to 15 years. The cases were divided in 4 groups of age: 0-12 month (Group I), 13 month-6 year (Group II), 7-11 year (Group III), and 12-15 year (Group IV). Mean cholesterol, triglyceride, HDL-C, LDL-C and VLDL-C levels were 102.01 +/- 27.91, 96.32 +/- 37.57, 32.13 +/- 11.69, 46.64 +/- 21.97 and 24.19m +/- 10.56 in group I, 142. 58 +/- 30.12, 91.57 +/- 37.27, 45.68 +/- 13.86, 78.41 +/- 32.23 and 21.55 +/- 10.75 in group II, 139.85 +/- 28.06, 95.42 +/- 43.12, 46.04 +/- 14.03, 71.44 +/- 27.05 and 21.69 +/- 12.51 in group III, and 153.98 +/- 26.71, 101.72 +/- 46.66, 45.84 +/- 13.06, 83.33 +/- 27.25 and 25.17 +/- 12.66 in group IV, respectively. There is no significant difference in serum lipid levels between males and females in any age groups. Mean values of cholesterol, HDL-C and LDL-C are significantly higher in older age group.