Suarez Edward C, Lewis James G, Krishnan Ranga R, Young Kenneth H
Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, P.O. Box 3328, Durham, NC 27710, USA.
Psychoneuroendocrinology. 2004 Oct;29(9):1119-28. doi: 10.1016/j.psyneuen.2004.01.002.
The current study investigated the relation of hostility and severity of depressive symptoms, separately and jointly, to the capacity of blood monocytes to secrete an array of cytokines when stimulated by bacterial lipopolysaccharide (LPS). Subjects were 44 healthy, non-smoking, premenopausal women (aged 23-49 years) not currently taking oral contraceptives. Data were collected during the follicular phase of the menstrual cycle. The Cook-Medley Hostility (Ho) scale and the Beck Depression Inventory (BDI) were used to assess hostility and severity of depressive symptoms, respectively. Dual-color flow cytometry was used to measure the total expression of interleukin (IL)-1alpha, IL-1beta, IL-8, tumor necrosis factor (TNF)-alpha, monocyte chemotactic protein (MCP)-1 and monocyte inflammatory protein (MIP)-1alpha in blood monocytes following 4 h in vitro LPS stimulation of whole blood. In analyses adjusting for age, body mass index (BMI), fasting cholesterol, alcohol use, race and 17beta-estradiol (E(2)), higher Ho scores were associated with greater LPS-stimulated expression of IL-1alpha (beta = 0.033, p = 0.02), IL-8 (beta = 0.046, p = 0.01) and IL-1beta (beta = 0.024, p = 0.06). Higher BDI scores were associated with greater expression of TNF-alpha (beta = 0.042, p = 0.02) and IL-8 (beta = 0.045, p = 0.04). The linear combination of Ho and BDI scores was significantly associated with IL-1beta (beta = 0.18, p = 0.057), IL-8 (beta = 0.36, p = 0.01), TNF-alpha (beta = 0.25, p = 0.03), and IL-1alpha (beta = 0.18, p < 0.07). Thus, in healthy women, these psychological risk factors, alone and in combination, induce a proinflammatory phenotype in circulating monocytes characterized by the up-regulation of proinflammatory cytokines, supporting the hypothesis that inflammation may be a key pathway whereby hostility and depressive symptoms contribute to atherosclerosis and subsequent coronary heart disease (CHD).
本研究分别及联合调查了敌意和抑郁症状严重程度与血液单核细胞在受到细菌脂多糖（LPS）刺激时分泌一系列细胞因子能力之间的关系。研究对象为44名健康、不吸烟、未绝经且目前未服用口服避孕药的女性（年龄23 - 49岁）。数据在月经周期的卵泡期收集。分别使用库克 - 梅德利敌意（Ho）量表和贝克抑郁量表（BDI）来评估敌意和抑郁症状的严重程度。采用双色流式细胞术测量全血在体外经LPS刺激4小时后血液单核细胞中白细胞介素（IL）-1α、IL - 1β、IL - 8、肿瘤坏死因子（TNF）-α、单核细胞趋化蛋白（MCP）-1和单核细胞炎症蛋白（MIP）-1α的总表达量。在对年龄、体重指数（BMI）、空腹胆固醇、饮酒情况、种族和17β - 雌二醇（E₂）进行校正的分析中，较高的Ho得分与LPS刺激后IL - 1α（β = 0.033，p = 0.02）、IL - 8（β = 0.046，p = 0.01）和IL - 1β（β = 0.024，p = 0.06）的表达增加相关。较高的BDI得分与TNF - α（β = 0.042，p = 0.02）和IL - 8（β = 0.045，p = 0.04）的表达增加相关。Ho和BDI得分的线性组合与IL - 1β（β = 0.18，p = 0.057）、IL - 8（β = 0.36，p = 0.01）、TNF - α（β = 0.25，p = 0.03）和IL - 1α（β = 0.18，p < 0.07）显著相关。因此，在健康女性中，这些心理风险因素单独或共同作用，会在循环单核细胞中诱导出一种以促炎细胞因子上调为特征的促炎表型，支持了炎症可能是敌意和抑郁症状导致动脉粥样硬化及随后冠心病（CHD）的关键途径这一假说。
