Rogers Sharon A, Liapis Helen, Hammerman Marc R
Renal Division, Department of Medicine, Washington University School of Medicine, St. Louis MO 63110, USA.
Transpl Immunol. 2005 Jun;14(2):67-75. doi: 10.1016/j.trim.2005.02.004.
Embryonic day (E) 28 (E28) pig pancreatic anlagen (PPA) transplanted into the omentum of non-immunosuppressed steptozotocin-diabetic Lewis rats normalize levels of circulating glucose within 2-4 weeks. Following transplantation formerly diabetic rats have porcine insulin, but no rat insulin detectable in circulation. At 3 weeks post-E28 PPA transplantation, bits of insulin-positive tissue are observed amidst host omental fat, but by 6 weeks only individual alpha and beta cells remain. In contrast, E35 PPA transplantation does not normalize glucose and 6 weeks post-implantation of E35 PPA transplanted tissue is rejected. In contrast to E28 PPA, no trace of implanted renal tissue is detectable post implantation of E28 pig renal anlagen (PRA) in non-immunosuppressed non-diabetic rats or in streptozocin-diabetic rats previously transplanted with E28 PPA. In the latter, normoglycemia is maintained post-PRA transplantation. We conclude that normalization of glucose levels following transplantation of PPA into non-immunosuppressed Lewis rats depends on obtaining the anlagen before E35 and that prior successful engraftment of E28 PPA as reflected by normalization of glucose, does not permit successful engraftment of E28 PRA.
将胚胎日（E）28（E28）猪胰腺原基（PPA）移植到未免疫抑制的链脲佐菌素诱导的糖尿病Lewis大鼠的大网膜中，可在2 - 4周内使循环葡萄糖水平恢复正常。移植后，先前的糖尿病大鼠体内有猪胰岛素，但循环中未检测到大鼠胰岛素。在E28 PPA移植后3周，在宿主大网膜脂肪中观察到胰岛素阳性组织小块，但到6周时仅剩下单个的α和β细胞。相比之下，E35 PPA移植不能使葡萄糖水平恢复正常，且E35 PPA移植组织在植入后6周被排斥。与E28 PPA不同，将E28猪肾原基（PRA）植入未免疫抑制的非糖尿病大鼠或先前已移植E28 PPA的链脲佐菌素诱导的糖尿病大鼠后，未检测到植入肾组织的痕迹。在后者中，PRA移植后血糖正常得以维持。我们得出结论，将PPA移植到未免疫抑制的Lewis大鼠后葡萄糖水平恢复正常取决于在E35之前获取原基，并且如葡萄糖恢复正常所反映的，先前成功植入E28 PPA并不允许E28 PRA成功植入。
