Huang Qian, Zhou Xue, Ding Yan, Peng Jin, Zhao Wei, Zhang Wei
Department of Histology, Embryology and Neurobiolgy, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 May;37(3):381-3, 403.
To investigate the impact of acupuncture on NP-1 expression in spared dorsal root ganglion (DRG).
Twenty-five male adult cats were divided into five groups. The five cats in the pseudo-operation group were kept intact. Ten cats were subjected to unilateral root rhizotomy(unilateral L1-L5 DRGs were transected, but L6 DRG was spared), with five sacrificed seven days later and another five sacrificed 14 days later. Ten cats with unilateral root rhizotomy received acupuncture (electron-needle stimulation in Zusanli, Xuanzhong, Futu and Sanyinjiao which are located in the distribution area of L6 spinal nerve) for 30 minutes per day. Five cats in the acupuncture group were sacrificed seven days and 14 days later respectively. The L6 DRG from the experimental side of each animal was taken and made into frozen sections 20 microm in thickness. The sections were stained under the same conditions using NP-1 specific antibody (1:600, Santa) by immunohistochemistry ABC method. The amounts of NP-1 immunoreaction neurons of DRG were measured.
The NP-1 immunoreactants were mainly located in the small and medium-sized neurons and a few large sized neurons in the cats of the pseudo-operation group. Less NP-1 positive medium-small sized neurons were found seven days after partial dorsal root rhizotomy (P<0.05). But they returned back to the original level 14 days after operation. The NP-1 positive large neurons had no significant changes after operations. With seven days of acupuncture, the amounts of NP-1 positive small and medium-sized neurons increased significantly (P<0.05) but were still less than the original level (P<0.05). There were no differences in neurons 14 days after operations between the acupuncture and non-acupuncture group (P>0.05) and between the groups with seven days acupuncture and 14 days of acupuncture (P>0.05).
Acupuncture increases the expression of NP-1 in small and medium-sized neurons of spared DRG.
探讨针刺对保留背根神经节（DRG）中NP-1表达的影响。
将25只成年雄性猫分为五组。假手术组的5只猫保持完整。10只猫接受单侧神经根切断术（切断单侧L1-L5 DRG，但保留L6 DRG），其中5只在7天后处死，另外5只在14天后处死。10只接受单侧神经根切断术的猫每天接受针刺治疗（在位于L6脊神经分布区域的足三里、悬钟、扶突和三阴交进行电针刺激）30分钟。针刺组的5只猫分别在7天和14天后处死。取每只动物实验侧的L6 DRG制成20微米厚的冰冻切片。切片在相同条件下用NP-1特异性抗体（1:600，Santa）通过免疫组织化学ABC法染色。测量DRG中NP-1免疫反应性神经元的数量。
假手术组猫的NP-1免疫反应物主要位于中小神经元和少数大神经元中。部分背根切断术后7天，NP-1阳性中小神经元数量减少（P<0.05）。但术后14天恢复到原来水平。术后NP-1阳性大神经元无明显变化。针刺7天后，NP-1阳性中小神经元数量显著增加（P<0.05），但仍低于原来水平（P<0.05）。针刺组和非针刺组术后14天的神经元数量无差异（P>0.05），针刺7天组和针刺14天组之间也无差异（P>0.05）。
针刺可增加保留DRG中小神经元中NP-1的表达。
Zotero 插件