Lee Byung Cheol, Lee Hye-jung, Chung Joo-Ho
Department of Oriental Internal Medicine, Kyung Hee University, Seoul, Republic of Korea.
Neurosci Lett. 2006 Dec 20;410(2):141-5. doi: 10.1016/j.neulet.2006.08.024. Epub 2006 Nov 3.
Peroxisome proliferator-activated receptor (PPAR)-gamma2 has important effects to insulin sensitivity, atherosclerosis, inflammation and endothelial cell function. Through these effects, PPAR-gamma2 might be involved with the ischemic stroke in type 2 diabetes. To determine the role of PPAR-gamma2 in genetic susceptibility to ischemic stroke in type 2 diabetes, we genotyped 302 ischemic stroke patients, 283 healthy controls and 141 type 2 diabetic patients without ischemic stroke (diabetes duration >10 years) for PPAR-gamma2 Pro12Ala polymorphism by polymerase chain reaction-restriction fragment length polymorphism methods. PPAR-gamma2 Pro/Ala genotype were lower in ischemic stroke patients than those observed in the control group (4.0% vs. 9.9%, OR=0.38, P=0.0046), and it were associated with the incidence of ischemic stroke in the multivariate analysis (OR=0.43, P=0.025). Genotypic analysis revealed that ischemic stroke patients with type 2 diabetes displayed a great lower prevalence of the Pro/Ala genotype (2.3%) than controls (9.9%) (OR=0.21, P=0.0047). And Pro/Ala genotype of type 2 diabetes patients with ischemic stroke were lower than type 2 diabetes patients without ischemic stroke (2.3% vs. 8.5%, OR=0.25, P=0.0321), however the significant association with ischemic stroke was not detected in the multivariate analysis (OR=0.27, P=0.051). These results suggest that the Pro/Ala genotype of PPAR-gamma2 Pro12Ala polymorphism may be associated with reduced risk for ischemic stroke, and the possibility that it might have a protective effect for ischemic stroke with type 2 diabetes.
过氧化物酶体增殖物激活受体（PPAR）-γ2对胰岛素敏感性、动脉粥样硬化、炎症及内皮细胞功能具有重要影响。通过这些作用，PPAR-γ2可能与2型糖尿病患者的缺血性脑卒中有关。为确定PPAR-γ2在2型糖尿病患者缺血性脑卒中遗传易感性中的作用，我们采用聚合酶链反应-限制性片段长度多态性方法，对302例缺血性脑卒中患者、283例健康对照者及141例无缺血性脑卒中的2型糖尿病患者（糖尿病病程>10年）进行了PPAR-γ2 Pro12Ala多态性基因分型。缺血性脑卒中患者的PPAR-γ2 Pro/Ala基因型低于对照组（4.0% 对9.9%，OR=0.38，P=0.0046），多因素分析显示其与缺血性脑卒中的发生率相关（OR=0.43，P=0.025）。基因型分析显示，2型糖尿病缺血性脑卒中患者的Pro/Ala基因型患病率（2.3%）显著低于对照组（9.9%）（OR=0.21，P=0.0047）。2型糖尿病缺血性脑卒中患者的Pro/Ala基因型低于无缺血性脑卒中的2型糖尿病患者（2.3% 对8.5%，OR=0.25，P=0.0321），然而多因素分析未发现其与缺血性脑卒中存在显著关联（OR=0.27，P=0.051）。这些结果提示，PPAR-γ2 Pro12Ala多态性的Pro/Ala基因型可能与缺血性脑卒中风险降低有关，且可能对2型糖尿病患者的缺血性脑卒中具有保护作用。
