肝细胞生长因子与表皮生长因子对大鼠肝细胞内钙离子作用的比较

Comparison of effects of HGF and EGF on cellular calcium in rat hepatocytes.

作者信息

Mine T, Kojima I, Ogata E, Nakamura T

机构信息

Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Dec 31;181(3):1173-80. doi: 10.1016/0006-291x(91)92062-o.

DOI:10.1016/0006-291x(91)92062-o

PMID:1837217

Abstract

We compared the effects of HGF and EGF on cytoplasmic free calcium concentration, [Ca2+]c, and inositol trisphosphate production in rat hepatocytes. HGF induced a prompt and transient elevation of [Ca2+]c. EGF also induced an immediate increase in [Ca2+]c, the magnitude of which was greater than that by HGF. In contrast, in the presence of 1 microM extracellular calcium EGF increased [Ca2+]c to a lesser extent than HGF. When cells were pretreated with EGF, the effect of HGF on [Ca2+]c was greatly enhanced. However, such enhancement was not observed in medium containing 1 microM extracellular calcium. In hepatocytes prelabeled with [3H]-inositol, both HGF and EGF increased [3H]inositol trisphosphate. HGF and EGF acted synergistically to stimulate production of inositol trisphosphate. These results indicate that both HGF and EGF increase [Ca2+]c by a mechanism involving phosphoinositide turnover and that the actions of HGF and EGF on hepatocyte calcium metabolism are not totally identical.

摘要

我们比较了肝细胞生长因子（HGF）和表皮生长因子（EGF）对大鼠肝细胞胞质游离钙浓度（[Ca2+]c）及三磷酸肌醇生成的影响。HGF可引起[Ca2+]c迅速且短暂的升高。EGF也可使[Ca2+]c立即升高，其升高幅度大于HGF所致的升高幅度。相反，在存在1微摩尔细胞外钙的情况下，EGF使[Ca2+]c升高的程度小于HGF。当细胞用EGF预处理后，HGF对[Ca2+]c的作用显著增强。然而，在含有1微摩尔细胞外钙的培养基中未观察到这种增强作用。在用[3H] - 肌醇预标记的肝细胞中，HGF和EGF均能增加[3H]肌醇三磷酸。HGF和EGF协同作用以刺激肌醇三磷酸的生成。这些结果表明，HGF和EGF均通过涉及磷酸肌醇代谢的机制增加[Ca2+]c，且HGF和EGF对肝细胞钙代谢的作用并非完全相同。